Effects of a calcium chelator, a detergent and the soybean lecithin on the quality of the frozen-thawed goat semen

Authors

  • Rodrigo Freitas Bittencourt Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP
  • Antônio de Lisboa Ribeiro Filho Universidade Federal da Bahia, Escola de Medicina Veterinária, Salvador, BA
  • Marcos Chalhoub Coelho Lima Universidade Federal da Bahia, Escola de Medicina Veterinária, Salvador, BA
  • Sidney Gonçalves Gonzalez Alves Universidade Federal da Bahia, Escola de Medicina Veterinária, Salvador, BA
  • Marta Freitas Vasconcelos Universidade de Brasília, Faculdade de Medicina Veterinária, Brasília, DF
  • Carmo Emanuel Biscarde Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP
  • Luciana da Silva Leal Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP
  • Eunice Oba Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2008.26690

Keywords:

Goat, Semen, Cryopreservation, Extender

Abstract

The aim of this paper was verifying the effect of the Equex STM Paste and EDTA addition to a Tris-egg yolk extender, on the post-thaw goat sperm viability. Nine semen samples of two adult goats were collected by artificial vagina and cryopreserved. It was also objective of this study, to evaluate the utilization of a soybean lecithin based commercial extender (Bioexcell® - IMV, L'Aigle, French) for the goat semen freezing. They were formed five experimental groups: TRIS; TRIS+EDTA; TRIS+EQUEX; TRIS+EDTA+EQUEX e Bioexcell. After evaluation, the semen was diluted in the five extenders and packed in 0.25mL straws with 100 million of motile spermatozoa. The samples were cooled at 0,46°C/min to 5°C, submitted at 75min of equilibration time and frozen in liquid nitrogen vapour. The thawing was accomplished in 37ºC water bath for 50s. There were no differences (P>;0,05) on the means of post-thaw total and progressive sperm motility among the groups TRIS, TRIS+EQUEX and TRIS+EQUEX+EDTA. The Bioexcell group obtained the least (P<0,05) percentage of post-thawing total and progressive sperm motility. After the thermotolerance test, it was observed the greatest (P<0,05) rates of total and progressive sperm motility in the Equex STM groups (TRIS+EQUEX and TRIS+EQUEX+EDTA). Thus, it can be affirmed that the Equex addition promotes better maintenance rates in the pos-thaw sperm viability, when compared with the extenders that did not contain it.

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Published

2008-08-01

Issue

Section

UNDEFINIED

How to Cite

Effects of a calcium chelator, a detergent and the soybean lecithin on the quality of the frozen-thawed goat semen. (2008). Brazilian Journal of Veterinary Research and Animal Science, 45(4), 305-312. https://doi.org/10.11606/issn.1678-4456.bjvras.2008.26690