Immunogenicity evaluation of proteins from a Mycobacterium bovis isolated from cattle in the State of Goiás, Brasil

Abstract

Bovine tuberculosis, persists in several countries as a threat to health of the herds despite the efforts given to the control and eradication of the disease. In order to detect immunogenic proteins with a potential to be used in a bovine tuberculosis diagnosis test, serum samples obtained from reactors and no reactors to intradermal tuberculin test, were evaluated for reactivity to Mycobacterium bovis antigens from a bacilli originated in Goiás – Brazil. The proteins immunogenicity from M. bovis was obtained by Western blot according to the molecular weight profile. The majority of the PPD positive bovine (67.92%) recognized a protein with 26kDa, suggesting the use of this protein in a serological test for bovine tuberculosis.