Usefulness of aqueous and vitreous humor analysis in infectious uveitis




Polymerase Chain Reaction, Infectious Disease, Uveitis, Aqueous Humor, Vitreous Humor


OBJECTIVE: To evaluate the role of intraocular fluid analysis as a diagnostic aid for uveitis. METHODS: Twenty-eight samples (27 patients including 3 HIV-infected patients) with active (n=24) or non-active (n=4) uveitis were submitted to aqueous (AH; n=12) or vitreous humor (VH) analysis (n=16). All samples were analyzed by quantitative PCR for herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV) and Toxoplasma gondii. RESULTS: The positivity of the PCR in AH was 41.7% (5/12), with 50% (2/4) in immunocompetent and 67% (2/3) in HIV+ patients. The positivity of the PCR in VH was 31.2% (5/16), with 13% (1/8) in immunocompetent and 50% (4/8) in immunosuppressed HIV negative patients. The analysis was a determinant in the diagnostic definition in 58% of HA and 50% of VH. CONCLUSION: Even in posterior uveitis, initial AH analysis may be helpful. A careful formulation of possible clinical diagnosis seems to increase the chance of intraocular sample analysis being meaningful.


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How to Cite

Santos, H. N. V. dos, Ferracioli-Oda, E., Barbosa, T. S., Otani, C. S. V., Tanaka, T., Silva, L. de C. S. da, Lopes, G. de O., Doi, A., Hirata, C. E., & Yamamoto, J. H. (2020). Usefulness of aqueous and vitreous humor analysis in infectious uveitis. Clinics, 75, e1498.



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