Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina

Authors

  • Sudong Liu Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou People’s Hospital (Huangtang Hospital), Research Experimental Center
  • Xuemin Guo Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou People’s Hospital (Huangtang Hospital), Research Experimental Center
  • Wei Zhong Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou People’s Hospital (Huangtang Hospital), Center for Cardiovascular Diseases
  • Ruiqiang Weng Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou People’s Hospital (Huangtang Hospital), Research Experimental Center
  • Jing Liu Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou People’s Hospital (Huangtang Hospital), Research Experimental Center
  • Xiaodong Gu Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou People’s Hospital (Huangtang Hospital), Research Experimental Center
  • Zhixiong Zhong Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou People’s Hospital (Huangtang Hospital), Center for Cardiovascular Diseases https://orcid.org/0000-0002-3200-3105

DOI:

https://doi.org/10.6061/clinics/2020/e1546

Keywords:

Coronary Artery Disease, MicroRNA (miRNA), Stable Angina, Unstable Angina, RNA sequencing (RNA-seq)

Abstract

OBJECTIVES: High incidence and case fatality of unstable angina (UA) is, to a large extent, a consequence of the lack of highly sensitive and specific non-invasive markers. Circulating microRNAs (miRNAs) have been widely recommended as potential biomarkers for numerous diseases. In the present study, we characterized distinctive miRNA expression profiles in patients with stable angina (SA), UA, and normal coronary arteries (NCA), and identified promising candidates for UA diagnosis. METHODS: Serum was collected from patients with SA, UA, and NCA who visited the Department of Cardiovascular Diseases of the Meizhou People’s Hospital. Small RNA sequencing was carried out on an Illumina HiSeq 2500 platform. miRNA expression in different groups of patients was profiled and then confirmed based on that in an independent set of patients. Functions of differentially expressed miRNAs were predicted using gene ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis. RESULTS: Our results indicated that circulating miRNA expression profiles differed between SA, UA, and NCA patients. A total of 36 and 161 miRNAs were dysregulated in SA and UA patients, respectively. miRNA expression was validated by reverse transcription quantitative polymerase chain reaction. CONCLUSION: The results suggest that circulating miRNAs are potential biomarkers of UA.

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Published

2020-07-27

How to Cite

Liu, S., Guo, X., Zhong, W., Weng, R., Liu, J., Gu, X., & Zhong, Z. (2020). Circulating MicroRNA Expression Profiles in Patients with Stable and Unstable Angina. Clinics, 75, e1546. https://doi.org/10.6061/clinics/2020/e1546

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Section

Original Articles