Histological study of fresh versus frozen semitendinous muscle tendon allografts

Authors

  • Alexandre Carneiro Bitar Universidade de São Paulo; Faculdade de Medicina; Hospital das Clinicas; Institute of Orthopedics and Traumatology
  • Luiz Augusto Ubirajara Santos Universidade de São Paulo; Faculdade de Medicina; Hospital das Clinicas; Institute of Orthopedics and Traumatology
  • Alberto Tesconi Croci Universidade de São Paulo; Faculdade de Medicina; Hospital das Clinicas; Institute of Orthopedics and Traumatology
  • João Alberto Ramos Maradei Pereira Universidade de São Paulo; Faculdade de Medicina; Hospital das Clinicas; Institute of Orthopedics and Traumatology
  • Edgard N. França Bisneto Universidade de São Paulo; Faculdade de Medicina; Hospital das Clinicas; Institute of Orthopedics and Traumatology
  • Arlete Mazzini Miranda Giovani Universidade de São Paulo; Faculdade de Medicina; Hospital das Clinicas; Institute of Orthopedics and Traumatology
  • Claudia Regina G. C. M. Oliveira Universidade de São Paulo; Faculdade de Medicina; Hospital das Clinicas; Institute of Orthopedics and Traumatology

DOI:

https://doi.org/10.1590/S1807-59322010000300010

Keywords:

Cryopreservation, Tissue banks, Histology, Tendons, Tissue transplantation

Abstract

OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at -80°C in comparison to a control group kept at only -4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at -80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh". RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80ºC without suffering histological modifications.

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Published

2010-01-01

Issue

Vol. 65 No. 3 (2010)

Section

Basic Research

How to Cite

Histological study of fresh versus frozen semitendinous muscle tendon allografts . (2010). Clinics, 65(3), 297-303. https://doi.org/10.1590/S1807-59322010000300010