Protective effect of sildenafil citrate on contralateral testis injury after unilateral testicular torsion/detorsion

  • Hamit Yíldíz Fírat University; Faculty of Veterinary Medicine; Department of Obstetrics and Gynecology
  • Ali Said Durmus Fírat University; Faculty of Veterinary Medicine; Department of Surgery
  • Halil Şimşek University of Bingöl; Sanitary Services Vocational School of Higher Education
  • Mine Yaman University of Firat; Faculty of Veterinary Medicine; Department of Histology
Keywords: Testicular torsion, Ischemia-reperfusion damage, Sildenafil citrate, Rat

Abstract

OBJECTIVES: This study was designed to investigate prevention of contralateral testicular injury with sildenafil citrate after unilateral testicular torsion/detorsion. METHODS: Thirty-seven adult male rats were divided into four groups: sham operated (group 1, n = 7), torsion/detorsion + saline (group 2, n = 10), torsion/detorsion + 0.7 mg of sildenafil citrate (group 3, n = 10) and torsion/detorsion + 1.4 mg of sildenafil citrate (group 4, n = 10). Unilateral testicular torsion was created by rotating the right testis 720º in a clockwise direction for 2 h in other groups, except for group 1, which was served as sham group. After torsion (2 h) and detorsion (2 h) periods, rats were killed. RESULTS: The level of reduced glutathion (GSH) (p<0.05) and the activities of catalase (p<0.01) and glutathione peroxidase (p<0.05) in the contralateral testis from group 2 were significantly lower and nitric oxide (NO) (p<0.05) level in the contralateral testis were significantly higher than those of group 1. Administration of low-dose sildenafil citrate (group 3) prevented the increases in malondialdehyde and NO levels and decreases in glutathione peroxidase activities and GSH values induced by testicular torsion. However, administration of high-dose sildenafil citrate (group 4) had no effect on these testicular parameters (p>0.05). Histopathological changes were detected in groups 2, 3 and 4. CONCLUSION: These results suggest that biochemically and histologically torsion/detorsion injury occurs in the contralateral testis following 2-h torsion and 2-h detorsion and that administration of low-dose sildenafil citrate before detorsion prevents ischemia/reperfusion cellular damage in testicular tissue.

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Published
2011-01-01
How to Cite
Yíldíz, H., Durmus, A., &#350;im&#351;ekH., & Yaman, M. (2011). Protective effect of sildenafil citrate on contralateral testis injury after unilateral testicular torsion/detorsion . Clinics, 66(1), 137-142. https://doi.org/10.1590/S1807-59322011000100024
Section
Basic Researches