Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses
DOI:
https://doi.org/10.6061/clinics/2014(04)07Abstract
OBJECTIVE: Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats. METHODS: Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g) treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal) or high-risk (ischemic) end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL) or low (40 mg/mL) concentrations and thrombin at high (1000 IU/mL) or low (500 IU/mL) concentrations. RESULTS: Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis. CONCLUSIONS: Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery.Downloads
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Published
2014-04-01
Issue
Section
Basic Researchs
How to Cite
Buen, E. P.- de, Orozco-Mosqueda, A., Leal-Cortés, C., Vázquez-Camacho, G., Fuentes-Orozco, C., Alvarez-Villaseñor, A. S., Macías-Amezcua, M. D., & González-Ojeda, A. (2014). Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses . Clinics, 69(4), 259-264. https://doi.org/10.6061/clinics/2014(04)07