Adhesion evaluation of Candida albicans on the surface of termopolymerizable acrylic resins treated with photopolymerizable coatings and/or silica nanoparticles addition

an in vitro study

Authors

  • Helidior Teixeira Lopes Centro Universitário da Serra Gaúcha
  • Liliane Alves Centro Universitário da Serra Gaúcha
  • Alexandra Flávia Gazzoni Centro Universitário da Serra Gaúcha

DOI:

https://doi.org/10.11606/issn.2357-8041.clrd.2019.157790

Keywords:

Oral Candidiasis, Acrylic Resins, Cell Adhesion, Silica

Abstract

To evaluate how the addition of 0.5% silica nanoparticles and/or photopolymerizable coatings affect the adhesion of Candida albicans on the surface of acrylic resin PMMA (poly-methyl methacrylate). Materials and methods: A total of 40 specimens were fabricated (65 mm × 10 mm × 2,5 mm) and randomly divided into four experimental groups according to surface treatment using silica and/or photopolymerizable coating: the control group (G1); silica group (G2) (0.5% silica); photopolymerizable coating group (G3); and silica group (0.5% silica) + photopolymerizable coatings (G4). Specimens were separately incubated with a Candida albicans suspension spectrophotometrically standardized with 1 × 107 CFU/ml at 37 °C for 90 minutes. After this period, the specimens were removed and then their fungal adhesion was analyzed by the colonyforming units counting. Data were analyzed by the One-way ANOVA test followed by the Tukey test (P ≤ 0,05). Results: A significant reduction in Candida albicans adhesion was observed in G2, G3, and G4 when compared to the control group G1 (P ≤ 0.0001). However, similar fungal adhesion was observed when G2, G3, and G4 were compared (P ≤ 0.05). Conclusions: The addition of silica nanoparticles and/or photopolymerizable coating on the surface of PMMA decreases the adhesion of Candida albicans, which can be used as a strategy to reduce oral lesions caused by acrylic dentures.

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Author Biographies

  • Helidior Teixeira Lopes, Centro Universitário da Serra Gaúcha

    Departamento de Odontologia, Centro Universitário da Serra Gaúcha (FSG), Caxias do Sul, RS, Brasil.

  • Liliane Alves, Centro Universitário da Serra Gaúcha

    Departamento de Odontologia, Centro Universitário da Serra Gaúcha (FSG), Caxias do Sul, RS, Brasil.

  • Alexandra Flávia Gazzoni, Centro Universitário da Serra Gaúcha

    Laboratório de Patologia e Microbiologia Oral, Centro Universitário da Serra Gaúcha (FSG), Caxias do Sul, RS, Brasil.

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Published

2019-12-06

Issue

2019

Section

Original Research

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