Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation

  • Ayşegül Mendi Gazi University; Faculty of Dentistry; Department of Medical Microbiology
  • Sevil Köse Hacettepe University; PEDI-STEM Center for Stem Cell Research and Development
  • Duygu Uçkan Hacettepe University; PEDI-STEM Center for Stem Cell Research and Development
  • Gülçin Akca Gazi University; Faculty of Dentistry; Department of Medical Microbiology
  • Derviş Yilmaz Gazi University; Faculty of Dentistry; Department of Oral and Maxilofacial Surgery
  • Levent Aral Gazi University; Faculty of Dentistry; Department of Oral and Maxilofacial Surgery
  • Sibel Elif Gültekin Gazi University; Faculty of Dentistry; Department of Oral Pathology
  • Tamer Eroğlu Başkent University; Faculty of Dentistry; Department of Oral and Maxillofacial Surgery
  • Emine Kiliç Technopolis of Hacettepe University; Department of Life Sciences
  • Sina Uçkan Başkent University; Faculty of Dentistry; Department of Oral and Maxillofacial Surgery

Abstract

An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits CXCL8 expression. Objective The aim of the study was to determine if P. gingivalis infection modulates the inflammatory response of gingival stromal stem cells (G-MSSCs), including the release of CXCL8, and the expression of TLRs and if immunomodulatory L. rhamnosus ATCC9595 could prevent CXCL8 inhibition in experimental inflammation. Material and Methods G-MSSCs were pretreated with L. rhamnosus ATCC9595 and then stimulated with P. gingivalis ATCC33277. CXCL8 and IL-10 levels were investigated with ELISA and the TLR-4 and 2 were determined through flow cytometer analysis. Results CXCL8 was suppressed by P. gingivalis and L. rhamnosus ATCC9595, whereas incubation with both strains did not abolish CXCL8. L. rhamnosus ATCC9595 scaled down the expression of TLR4 and induced TLR2 expression when exposed to P. gingivalis stimulation (p<0.01). Conclusions These findings provide evidence that L. rhamnosus ATCC9595 can modulate the inflammatory signals and could introduce P. gingivalis to immune systems by inducing CXCL8 secretion.

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Published
2016-02-01
Section
Original Articles