Liver proteome of mice with different genetic susceptibilities to the effects of fluoride

Authors

  • Zohaib Nisar KHAN Universidade de São Paulo; Faculdade de Odontologia de Bauru; Departamento de Ciências Biológicas
  • Aline de Lima LEITE Universidade Federal de São Carlos; Centro de Ciências Biológicas e da Saúde; Departamento de Genética e Evolução
  • Senda CHARONE Universidade de São Paulo; Faculdade de Odontologia de Bauru; Departamento de Ciências Biológicas
  • Isabela Tomazini SABINO Universidade de São Paulo; Faculdade de Odontologia de Bauru; Departamento de Ciências Biológicas
  • Tatiana MARTINI Universidade de São Paulo; Faculdade de Odontologia de Bauru; Departamento de Ciências Biológicas
  • Heloísa Aparecida Barbosa da Silva PEREIRA Universidade Federal de São Carlos; Centro de Ciências Biológicas e da Saúde; Departamento de Genética e Evolução
  • Rodrigo Cardoso OLIVEIRA Universidade de São Paulo; Faculdade de Odontologia de Bauru; Departamento de Ciências Biológicas
  • Marília Afonso Rabelo BUZALAF Universidade de São Paulo; Faculdade de Odontologia de Bauru; Departamento de Ciências Biológicas

DOI:

https://doi.org/10.1590/1678-775720150364

Abstract

A/J and 129P3/J mice strains have been widely studied over the last few years because they respond quite differently to fluoride (F) exposure. 129P3/J mice are remarkably resistant to the development of dental fluorosis, despite excreting less F in urine and having higher circulating F levels. These two strains also present different characteristics regardless of F exposure. Objective In this study, we investigated the differential pattern of protein expression in the liver of these mice to provide insights on why they have different responses to F. Material and Methods Weanling male A/J and 129P3/J mice (n=10 from each strain) were pared and housed in metabolic cages with ad libitum access to low-F food and deionized water for 42 days. Liver proteome profiles were examined using nLC-MS/MS. Protein function was classified by GO biological process (Cluego v2.0.7 + Clupedia v1.0.8) and protein-protein interaction network was constructed (PSICQUIC, Cytoscape). Results Most proteins with fold change were increased in A/J mice. The functional category with the highest percentage of altered genes was oxidation-reduction process (20%). Subnetwork analysis revealed that proteins with fold change interacted with Disks large homolog 4 and Calcium-activated potassium channel subunit alpha-1. A/J mice had an increase in proteins related to energy flux and oxidative stress. Conclusion This could be a possible explanation for the high susceptibility of these mice to the effects of F, since the exposure also induces oxidative stress.

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Published

2016-06-01

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Section

Original Articles