TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats

Authors

  • Bruna Vasconcelos OLIVEIRA Universidade Federal do Ceará; Setor de Patologia Oral; Departamento de Odontologia Clínica
  • Paulo Goberlânio BARROS SILVA Universidade Federal do Ceará; Setor de Patologia Oral; Departamento de Odontologia Clínica
  • Jacqueline de Santiago NOJOSA Universidade Federal do Ceará; Setor de Cariologia e Odontologia Restauradora; Departamento de Odontologia Restauradora
  • Luiz André Cavalcante BRIZENO Universidade Estadual do Ceará; Setor de Fisiologia e Farmacologia; Departamento de Ciências Biomédicas
  • Jamile Magalhães FERREIRA Universidade Federal do Ceará; Departamento de Fisiologia e Farmacologia
  • Fabrício Bitú SOUSA Universidade Federal do Ceará; Setor de Patologia Oral; Departamento de Odontologia Clínica
  • Mário Rogério Lima MOTA Universidade Federal do Ceará; Setor de Patologia Oral; Departamento de Odontologia Clínica
  • Ana Paula Negreiros Nunes ALVES Universidade Federal do Ceará; Setor de Patologia Oral; Departamento de Odontologia Clínica

DOI:

https://doi.org/10.1590/1678-775720150481

Abstract

Diabetes mellitus (DM) is a disease associated with delayed wound healing of oral ulcers by increased expression of proinflammatory cytokines and cellular apoptosis. Objective to evaluate the influence of Tumor Necrosis Factor alpha (TNF-α) and apoptosis in rats with DM treated with chamomile extract or triamcinolone. Material and Methods Wistar male rats (210.0±4.2 g) were divided into five groups: negative control group (NCG) without diabetes; positive control group (PCG) with DM (alloxan, 45 mg/kg); and groups treated with chamomile extract (normoglycemic= NCG group and diabetic= DCG group) and with triamcinolone (TG). Traumatic ulcers were performed on all animals that received topical triamcinolone, chamomile extract or saline 12/12 hours for ten days. Results On days five and ten the animals were euthanized and the ulcers were analyzed by light microscopy, TUNEL assay, and immunohistochemically (TNF-α). The NCG (p=0.0062), PCG (p=0.0285), NCG (p=0.0041), and DCG (p<0.0001) groups were completely healed on the 10th day, however, there was no healing on the TG (p=0.5127) group. The TNF-α expression showed a significant reduction from the 5th to the 10th day in NCG (p=0.0266) and DCG (p=0.0062). In connective tissue, the TUNEL assay showed a significant reduction in the number of positive cells in NCG (p=0.0273) and CNG (p=0.0469) and in the epithelium only in CDG (p=0.0320). Conclusions Chamomile extract can optimize the healing of traumatic oral ulcers in diabetic rats through the reduction of apoptosis in the epithelium and TNF-α expression.

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Published

2016-06-01

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Original Articles