Clinical trial for evaluation of Ricinus communis and sodium hypochlorite as denture cleanser

Authors

  • Maurício Malheiros BADARÓ Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Materiais Dentários e Prótese
  • Marcela Moreira SALLES Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Materiais Dentários e Prótese
  • Vanessa Maria Fagundes LEITE Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Materiais Dentários e Prótese
  • Carolina Noronha Ferraz de ARRUDA Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Materiais Dentários e Prótese
  • Viviane de Cássia OLIVEIRA Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Materiais Dentários e Prótese
  • Cássio do NASCIMENTO Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Materiais Dentários e Prótese
  • Raphael Freitas de SOUZA Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Materiais Dentários e Prótese
  • Helena de Freitas de Oliveira PARANHOS Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Materiais Dentários e Prótese
  • Cláudia Helena SILVA-LOVATO Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Materiais Dentários e Prótese

DOI:

https://doi.org/10.1590/1678-7757-2016-0222

Keywords:

Denture, Biofilms, Candidiasis, Disinfection

Abstract

The development of opportunistic infections due to poor denture hygiene conditions justified the search for effective hygiene protocols for controlling denture biofilm. Objective This study evaluated Ricinus communis and sodium hypochlorite solutions in terms of biofilm removal ability, remission of candidiasis, antimicrobial activity, and participant satisfaction. Material and Methods It was conducted a controlled clinical trial, randomized, double-blind, and crossover. Sixty-four denture wearers with (n=24) and without candidiasis (n=40) were instructed to brush (3 times/day) and immerse their dentures (20 min/day) in different storage solutions (S1 / S2: 0.25% / 0.5% sodium hypochlorite; S3: 10% R. communis; S4: Saline).The trial period for each solution was seven days and a washout period of seven days was used before starting the use of another solution. The variables were analyzed at baseline and after each trial period. The biofilm of inner surfaces of maxillary dentures was disclosed, photographed, and total and dyed areas were measured (Image Tool software). The percentage of biofilm was calculated. Remission of candidiasis was assessed by visual scale and score were attributed. Antimicrobial activity was assessed by the DNA-Checkerboard hybridization method. Patient satisfaction was measured using a questionnaire. Results S1 (4.41±7.98%) and S2 (2.93±5.23%) were more effective then S3 (6.95±10.93%) in biofilm remotion(P<0.0001). All solutions were different from the control (11.07±11.99%). S3 was the most effective solution in remission of candidiasis (50%), followed by S1 (46%). Concerning antimicrobial action, S1/S2 were similar and resulted in the lowest microorganism mean count (P=0.04), followed by S3. No significant differences were found with patient’s satisfaction. Conclusions 10% R. communis and 0.25% sodium hypochlorite were effective in biofilm removal, causing remission of candidiasis and reducing the formation of microbial colonies in denture surfaces. All solutions were approved by patients.

Downloads

Download data is not yet available.

Downloads

Published

2017-06-01

Issue

Section

Original Articles