Stanozolol promotes osteogenic gene expression and apposition of bone mineral in vitro

Authors

  • Giulia Ghiacci Università degli Studi di Parma, Dipartimento di Medicina e Chirurgia, Centro Universitario di Odontoiatria https://orcid.org/0000-0003-2902-2214
  • Simone Lumetti Università degli Studi di Parma, Dipartimento di Medicina e Chirurgia, Centro Universitario di Odontoiatria
  • Edoardo Manfredi Università degli Studi di Parma, Dipartimento di Medicina e Chirurgia, Centro Universitario di Odontoiatria
  • Daniele Mori Università degli Studi di Parma, Dipartimento di Medicina e Chirurgia, Unità di Patologia Generale https://orcid.org/0000-0003-4395-5686
  • Guido Maria Macaluso Università degli Studi di Parma, Dipartimento di Medicina e Chirurgia, Centro Universitario di Odontoiatria
  • Roberto Sala Università degli Studi di Parma, Dipartimento di Medicina e Chirurgia, Unità di Patologia Generale

DOI:

https://doi.org/10.1590/1678-7757-2018-0014

Keywords:

Osteogenesis, Bone matrix, Calcification, Gene expression, Androgens, Stanozolol

Abstract

Stanozolol (ST) is a synthetic androgen with high anabolic potential. Although it is known that androgens play a positive role in bone metabolism, ST action on bone cells has not been sufficiently tested to support its clinical use for bone augmentation procedures. Objective: This study aimed to assess the effects of ST on osteogenic activity and gene expression in SaOS-2 cells. Material and Methods: SaOS-2 deposition of mineralizing matrix in response to increasing doses of ST (0-1000 nM) was evaluated through Alizarin Red S and Calcein Green staining techniques at 6, 12 and 24 days. Gene expression of runt-related transcription factor 2 (RUNX2), vitamin D receptor (VDR), osteopontin (SPP1) and osteonectin (ON) was analyzed by RT-PCR. Results: ST significantly influenced SaOS-2 osteogenic activity: stainings showed the presence of rounded calcified nodules, which increased both in number and in size over time and depending on ST dose. RT-PCR highlighted ST modulation of genes related to osteogenic differentiation. Conclusions: This study provided encouraging results, showing ST promoted the osteogenic commitment of SaOS-2 cells. Further studies are required to validate these data in primary osteoblasts and to investigate ST molecular pathway of action.

Downloads

Download data is not yet available.

Downloads

Published

2019-05-29

Issue

Section

Original Articles