BK virus salivary shedding and viremia in renal transplant recipients

  • Dmitry José de Santana Sarmento University of São Paulo, School of Dentistry, Stomatology Department https://orcid.org/0000-0001-7972-9141
  • Michelle Palmieri University of São Paulo, School of Dentistry, Stomatology Department
  • Gustavo Souza Galvão University of São Paulo, School of Dentistry, Stomatology Department
  • Tânia Regina Tozetto-Mendoza University of São Paulo, Institute of Tropical Medicine of São Paulo, Laboratory of Virology
  • Cynthia Motta do Canto University of São Paulo, Institute of Tropical Medicine of São Paulo, Laboratory of Virology
  • Ligia Camera Pierrotti University of São Paulo, Medical School, Hospital das Clínicas, Departament of Infectious and Parasitic Diseases https://orcid.org/0000-0002-0216-3028
  • Elias David-Neto University of São Paulo, Medical School, Hospital das Clínicas, Serviço de Transplante Renal
  • Fabiana Agena University of São Paulo, Medical School, Hospital das Clínicas, Serviço de Transplante Renal https://orcid.org/0000-0002-4526-4857
  • Marina Gallottini University of São Paulo, School of Dentistry, Stomatology Department https://orcid.org/0000-0001-6071-5110
  • Claudio Sergio Pannuti University of São Paulo, Institute of Tropical Medicine of São Paulo, Laboratory of Virology https://orcid.org/0000-0003-4181-3975
  • Maria Cristina Domingues Fink University of São Paulo, Institute of Tropical Medicine of São Paulo, Laboratory of Virology
  • Paulo Henrique Braz-Silva University of São Paulo, School of Dentistry, Stomatology Department https://orcid.org/0000-0002-1842-9521
Keywords: Transplantation, BK virus, Saliva, Immunocompromised host, Polymerase chain reaction

Abstract

Objectives: This study aimed to verify the presence of polyomavirus BK (BKPyV) in the saliva of kidney transplant recipients and to correlate it with blood viremia. Material and Methods: We have conducted a crosssectional study with a sample involving 126 renal transplant recipients. 126 samples of saliva and 52 samples of blood were collected from these patients. Detection and quantification of BKPyV were performed using a real-time PCR. To compare the presence of BKPyV in blood and saliva, the binomial proportion test was used. To verify associations between salivary shedding BKPyV and post-transplant periods (in months), the Mann-Whitney test was used. Spearman’s correlation was used to correlate the viral load in the saliva with blood of kidney transplant recipients. Results: The mean age of the study group was 51.11±12.45 years old, and 69 participants (54.8%) were female, with a mean post-transplantation time of 4.80±6.04 months. BKPyV was quantified in several samples of saliva and blood, with medians of 1,108 cp/mL and 1,255 cp/mL, respectively. Only 16/52 (30.8%) participants presented BKPyV in blood, and 59/126 (46.8%) excreted the virus in saliva (p=0.004). BKPyV shedding was found in patients at a shorter post-transplantation period (3.86±5.25, p=0.100). A weak correlation was observed between viral quantification in saliva and blood (Spearman’s correlation coefficient=0.193). Conclusion: The results of this study suggested that, although saliva excretes more BKPyV than blood, there is no reliable correlation between salivary shedding and blood viremia, showing two independent compartments of viral replication.

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Published
2019-06-04
Section
Original Articles