Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy

Authors

  • Mariana Gouvêa Latini Abreu Universidade Federal Fluminense, Departamento de Odontologia, Área de Periodontia
  • Dione Kawamoto Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia https://orcid.org/0000-0002-6702-7000
  • Marcia Pinto Alves Mayer Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia https://orcid.org/0000-0002-5910-8433
  • Vinicius D’Avila Bitencourt Pascoal Universidade Federal Fluminense, Departamento de Ciências Básicas
  • Karina Sampaio Caiaffa Universidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araçatuba, Departamento de Endodontia https://orcid.org/0000-0002-8159-727X
  • Elizangela P. Zuza Universidade Federal Fluminense, Departamento de Odontologia, Área de Periodontia
  • Cristiane Duque Universidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araçatuba, Departamento de Odontopediatria e Saúde Pública https://orcid.org/0000-0002-2575-279X
  • Gabriela Alessandra da Cruz Galhardo Camargo Universidade Federal Fluminense, Departamento de Odontologia, Área de Periodontia https://orcid.org/0000-0002-0638-5509

DOI:

https://doi.org/10.1590/1678-7757-2018-0205

Keywords:

Periodontal disease, Smoking, Porphyromonas gingivalis

Abstract

Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.

Downloads

Download data is not yet available.

Downloads

Published

2019-06-04

Issue

Section

Original Articles