The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion

Authors

  • Laurent A. M. Thierens Ghent University, Department of Orthodontics, Oral Health Sciences https://orcid.org/0000-0002-3249-9198
  • Sophie Moerman Ghent University, Department of Orthodontics, Oral Health Sciences
  • Charlotte Van Elst Ghent University, Department of Orthodontics, Oral Health Sciences
  • Chris Vercruysse Ghent University, Department of Basic Medical Sciences, Biomaterials Group
  • Petra Maes Vrije Universiteit Brussel (VUB), Faculty of Medicine and Pharmacy, Oral Health Research Group (ORHE)
  • Liesbeth Temmerman Ghent University, Department of Orthodontics, Oral Health Sciences
  • Noëmi M. C. de Roo Ghent University, Department of Orthodontics, Oral Health Sciences https://orcid.org/0000-0002-7022-1680
  • Ronald M. H. Verbeeck Ghent University, Department of Basic Medical Sciences, Biomaterials Group
  • Guy A. M. de Pauw Ghent University, Department of Orthodontics, Oral Health Sciences

DOI:

https://doi.org/10.1590/1678-7757-2018-0589

Keywords:

Tooth remineralization, Dental white spots, In vitro

Abstract

Objective: The aim of this in vitro study was to determine the effects of remineralization promoting agents containing casein phosphopeptidestabilized amorphous calcium phosphate (CPP-ACP), or CPP-ACP in combination with fluoride (CPP-ACPF) on artificial white spot lesions (WSLs) after 6 and 12 weeks. Methodology: White spot lesions were created on 123 sectioned premolars (246 specimens) with a demineralization solution during a 96 hours pH-cycling regime. Two experimental groups were created: a CPP-ACP group (Tooth MousseTM), and a CPP-ACPF group (Mi Paste PlusTM). Additionally, two control groups were created, one using only a conventional toothpaste (1450 ppm fluoride) and another one without any working agents. All teeth were also daily brushed with the conventional toothpaste except the second control group. Tooth MousseTM and Mi Paste PlusTM were applied for 180 seconds every day. The volume of demineralization was measured with transverse microradiography. Six lesion characteristics regarding the lesion depth and mineral content of WSLs were also determined. Results: The application of CPP-ACP and CPP-ACPF had a significant regenerative effect on the WSLs. Compared to Control group 1 and 2 the volume of demineralization after 6 weeks decreased significantly for CPP-ACP (respectively p<0.001 and p<0.001) and CPP-ACPF (respectively p=0.001 and p=0.003). The same trend was observed after 12 weeks. For the CPP-ACPF group, WSL dimensions decreased significantly between 6 and 12 weeks follow-up (p=0.012). The lesion depth reduced significantly after application of CPP-ACP and CPP-ACPF but increased significantly in the Control groups. Mineral content increased for CPP-ACP and CPP-ACPF after an application period of 12 weeks, but this was only significant for CPP-ACP. Conclusions: Long-term use of CPP-ACP and CPP-ACPF in combination with a conventional tooth paste shows beneficial effects in the recovery of in vitro subsurface caries lesions.

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Published

2019-06-05

Issue

Section

Original Articles