Recombinant human insulin-like growth factor-1 promotes osteoclast formation and accelerates orthodontic tooth movement in rats

Authors

  • Ju-Xiang Peng Guiyang Stomatological Hospital Affiliated to Zunyi Medical University, Guiyang Hospital of Stomatology, Department of Orthodontic, Guiyang
  • Xiao-Yan Guan Guiyang Stomatological Hospital Affiliated to Zunyi Medical University, Guiyang Hospital of Stomatology, Department of Orthodontic, Guiyang
  • Gao-Hua Li Shenzhen Ai Kang Jian Stomatological Hospital, Outpatient Department of Stomatology, Shenzhen
  • Jian-Li Zhong Guangdong Province Stomatological Hospital, Department of Orthodontic, Guangzhou
  • Ju-Kun Song Guizhou Province People’s Hospital, Department of Oral and Maxillofacial Surgery, Guiyang
  • Lin-Lin Xiao Zunyi Medical University, School of Stomatology, Department of Orthodontic, Zunyi
  • Su-Han Jin Zunyi Medical University, School of Stomatology, Department of Orthodontic, Zunyi
  • Jian-Guo Liu Special Key Laboratory of Oral Diseases Research from Higher Education Institution of Guizhou Province & Zunyi Key Laboratory of Oral Disease Research, Zunyi http://orcid.org/0000-0001-8752-770X

DOI:

https://doi.org/10.1590/1678-7757-2020-0791

Keywords:

Insulin-like growth factor I, Osteoclasts, Orthodontics, Tooth movement techniques

Abstract

Background: IGF-1 may be an important factor in bone remodeling, but its mechanism of action on osteoclasts during orthodontic tooth movement is complex and unclear. Methodology: The closed-coil spring was placed between the left maxillary first molar and upper incisors with a force of 50 g to establish an orthodontic movement model. Eighty SD rats were randomized to receive phosphate buffer saline or 400 ng rhIGF-1 in the lateral buccal mucosa of the left maxillary first molar every two days. Tissue sections were stained for tartrate-resistant acidic phosphatase (TRAP), the number of TRAP-positive cells was estimated and tooth movement measured. Results: The rhIGF-1 group exhibited evidential bone resorption and lacuna appeared on the alveolar bone compared to the control group. Moreover, the number of osteoclasts in compression side of the periodontal ligament in the rhIGF-1 group peaked at day 4 (11.37±0.95 compared to 5.28±0.47 in the control group) after the orthodontic force was applied and was significantly higher than that of the control group (p<0.01). Furthermore, the distance of tooth movement in the rhIGF-1 group was significantly larger than that of the control group from day 4 to day 14 (p<0.01), suggesting that rhIGF-1 accelerated orthodontic tooth movement. Conclusion: Our study has showed that rhIGF-1 could stimulate the formation of osteoclasts in the periodontal ligament, and accelerate bone remodeling and orthodontic tooth movement.

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Published

2021-07-06

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Section

Original Articles