Bioactivity effects of extracellular matrix proteins on apical papilla cells

Authors

  • Maria Luísa Leite Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Materiais Odontológicos e Prótese, Araraquara http://orcid.org/0000-0001-6650-3637
  • Diana Gabriela Soares Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Dentística, Endodontia e Materiais Odontológicos, Bauru http://orcid.org/0000-0002-1485-6104
  • Giovana Anovazzi Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Morfologia e Clínica Infantil, Araraquara http://orcid.org/0000-0003-0164-0141
  • Filipe Koon Wu Mon Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Fisiologia e Patologia, Araraquara http://orcid.org/0000-0001-9317-0460
  • Ester Alves Ferreira Bordini Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Materiais Odontológicos e Prótese, Araraquara http://orcid.org/0000-0002-4178-5794
  • Josimeri Hebling Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Morfologia e Clínica Infantil, Araraquara http://orcid.org/0000-0002-2846-2325
  • Carlos Alberto De Souza Costa Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Fisiologia e Patologia, Araraquara http://orcid.org/0000-0002-7455-6867

DOI:

https://doi.org/10.1590/1678-7757-2021-0038

Keywords:

Guided tissue regeneration, Dental pulp, Fibronectin, Laminin, Collagen

Abstract

Potent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective: This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs). Methodology: Different concentrations (1, 5, and 10 µg/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5×103 cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1) (ANOVA/Tukey; α=0.05). Results: We observed greater attachment potential for cells on the FN substrate, with the effect depending on concentration. Concentrations of 5 and 10 µg/mL of FN yielded the highest cell proliferation, spreading and collagen synthesis values with 10 µg/mL concentration increasing the ITGA5, ITGAV, and COL1A1 expression compared with PC. LM (5 and 10 µg/mL) showed higher bioactivity values than NC, but those were lower than PC, and COL showed no bioactivity at all. Conclusion: We conclude that FN at 10 µg/mL concentration exerted the most intense bioactive effects on hAPCs.

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Published

2021-09-08

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Original Articles