Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge

Authors

  • Fabíola Galbiatti de Carvalho Universidade Federal de Juiz de Fora, Campus Governador Valadares, Departamento de Odontologia, Governador Valadares, Minas Gerais http://orcid.org/0000-0003-2510-1329
  • Eliseu Aldrighi Munchow Universidade Federal de Juiz de Fora, Campus Governador Valadares, Departamento de Odontologia, Governador Valadares, Minas Gerais
  • Hugo Lemes Carlo Universidade Federal de Juiz de Fora, Campus Governador Valadares, Departamento de Odontologia, Governador Valadares, Minas Gerais
  • Rogério Lacerda dos Santos Universidade Federal de Juiz de Fora, Campus Governador Valadares, Departamento de Odontologia, Governador Valadares, Minas Gerais http://orcid.org/0000-0002-6213-9206
  • Ângelo Márcio Leite Denadai Universidade Federal de Juiz de Fora, Campus Governador Valadares, Departamento de Farmácia, Governador Valadares, Minas Gerais
  • Renata Sobreira França Universidade Federal da Paraíba, Departamento de Odontologia Restauradora, Paraíba, João Pessoa
  • Natália Moreira Teixeira Universidade Federal de Juiz de Fora, Campus Governador Valadares, Departamento de Odontologia, Governador Valadares, Minas Gerais
  • Taís Chaves Magalhães Universidade Federal de Juiz de Fora, Campus Governador Valadares, Departamento de Odontologia, Governador Valadares, Minas Gerais http://orcid.org/0000-0003-1920-1745

DOI:

https://doi.org/10.1590/1678-7757-2021-0120

Keywords:

Chitosan, Nanoparticles, Dental caries, Dental enamel, Hardness

Abstract

Objective: Our study aims to synthesize, characterize, and determine the effects of a ChNPs suspension on human enamel after cariogenic challenge via pH-cycling. Methodology: ChNPs were synthesized by ion gelation and characterized by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering. Forty enamel blocks were divided into four groups (n=10/group): (i) ChNPs suspension; (ii) chitosan solution; (iii) 0.05% sodium fluoride (NaF) solution; and (iv) distilled water. Specimens were exposed to cariogenic challenge by cycling in demineralization solution (3 h) and then remineralized (21h) for 7 days. Before each demineralization cycle, the corresponding solutions were passively applied for 90 s. After 7 days, specimens were examined for surface roughness (Ra) and Knoop hardness (KHN) before and after the cariogenic challenge; % KHN change (variation between initial and final hardness), and surface topography by an optical profilometer. The data were analyzed by repeated-measures ANOVA, One-way ANOVA, and Tukey tests (α=0.05). Results: 
TEM images showed small spherical particles with diameter and zeta potential values of 79.3 nm and +47.9 mV, respectively. After the challenge, all groups showed an increase in Ra and a decrease in KHN values. Optical profilometry indicated that ChNPs- and NaF-treated specimens showed uneven roughness interspersed with smooth areas and the lowest %KHN values. Conclusion: The ChNPs suspension was successfully synthesized and minimized human enamel demineralization after a cariogenic challenge, showing an interesting potential for use as an oral formulation for caries prevention.

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Published

2021-10-14 — Updated on 2021-10-14

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