Quantitative analysis of neutrophils, mast cells and eosinophils in human prostatic adenocarcinoma
DOI:
https://doi.org/10.11606/issn.1679-9836.v102i5e-208256Keywords:
Prostate Cancer, Inflammation, Neutrophils, Mast Cells, EosinophilsAbstract
Objective: To evaluate the relationship between benign prostatic hyperplasia (BPH) and prostatic adenocarcinoma (AP) through the quantification of neutrophils, eosinophils and mast cells and to correlate with the histopathological grade of the neoplasm. Methodology: This is an observational cross-sectional study. Cataloged patient biopsies were sectioned into 5µm sections, stained and analyzed under a light microscope. In these sections, cells were quantified and compared between HPB and AP conditions by statistical analysis. Results: Biopsies of 47 patients were analyzed, 28 (59.6%) with BPH and 19 (40.4%) with AP. The median age of the BPH group was 69 years (range: 54 – 77 years) and the AP group was 66 years (range: 59 – 92 years). In the statistical analysis, a greater number of extravascular (p <0.001) and total (p <0.05) neutrophils was observed in the AP in relation to the BPH; however, there was no statistical difference between intravascular neutrophils, mast cells and eosinophils between the groups. By correlating the Gleason Score and the influx of inflammatory cells, it was observed that higher scores are associated with a lower influx of neutrophils and intact mast cells. In addition, it was observed that prostatic volume and weight with AP may be associated with inflammatory infiltrate. Conclusion: In this study, it was possible to suggest that prostate cancer is related to the innate immune response by the exacerbated influx of neutrophils in the tumor microenvironment and by the influence of these cells on the Gleason Score and on the values of weight and prostate volume. However, further studies are needed to better illustrate the role of neutrophils in tumorigenesis.
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