Prevalence of antinuclear autoantibodies in the serum of normal blood dornors

Authors

  • Solange Assuncion Villagra Fernandez University of São Paulo; Hospital das Clínicas; Immunopathology Laboratory; Department of Dermatology
  • Alice Zoghbi Coelho Lobo University of São Paulo; Hospital das Clínicas; Immunopathology Laboratory; Department of Dermatology
  • Zilda Najjar Prado de Oliveira University of São Paulo; Hospital das Clínicas; Immunopathology Laboratory; Department of Dermatology
  • Ligia Maria Ichimura Fukumori University of São Paulo; Hospital das Clínicas; Immunopathology Laboratory; Department of Dermatology
  • Alexandre Marques Périgo University of São Paulo; Hospital das Clínicas; Immunopathology Laboratory; Department of Dermatology
  • Evandro A. Rivitti University of São Paulo; Hospital das Clínicas; Immunopathology Laboratory; Department of Dermatology

DOI:

https://doi.org/10.1590/S0041-87812003000600005

Keywords:

Autoantibodies, Healthy population, Rheumatic diseases, Antinuclear antibodies, Autoimmunity

Abstract

OBJECTIVE:To examine the presence of serum antinuclear autoantibodies in a healthy population. METHODS: Serum of 500 normal blood donors between 18 and 60 years of age were tested for the presence of autoantibodies. Antinuclear antibodies were detected by indirect immunofluorescence technique using HEp-2 epithelial cells as the substrate. The presence of dnaN was detected by indirect immunofluorescence technique using Critidia lucillae as the substrate. Anti-SSA (RO), anti-SSB (LA), anti-Sm, and anti-RNP were determined by double radial immunodiffusion. RESULTS: In the evaluation of the presence of serum antibodies, antinuclear antibodies were detected in 22.6% of the sera. The presence of other antibodies was not significant. The majority of the titers were 1:40. CONCLUSION: The presence of autoantibodies is not necessarily pathologic and has to be related to the age group, gender, and clinical condition of the patient.

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Published

2003-01-01

Issue

Section

Original Articles

How to Cite

Prevalence of antinuclear autoantibodies in the serum of normal blood dornors . (2003). Revista Do Hospital Das Clínicas, 58(6), 315-319. https://doi.org/10.1590/S0041-87812003000600005