COMPARISON OF PERMANENT STAINING METHODS FOR THE LABORATORY DIAGNOSIS OF TRICHOMONIASIS

Authors

  • Camila Braz MENEZES Universidade Federal do Rio Grande do Sul; Faculdade de Farmácia
  • Mariana dos Santos MELLO Universidade Federal do Rio Grande do Sul; Faculdade de Farmácia
  • Tiana TASCA Universidade Federal do Rio Grande do Sul; Faculdade de Farmácia

Abstract

Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in the world. The diagnosis is based on wet mount preparation and direct microscopy on fixed and stained clinical specimens. The aim of this study was to compare the performance of different fixing and staining techniques used in the detection of T. vaginalis in urine. The smears were fixed and submitted to different methods of permanent staining and then, the morphological aspects of the parasites were analyzed and compared. The Papanicolaou staining with ethanol as the fixative solution showed to be the best method of permanent staining. Our data suggest that staining techniques in association with wet mount examination of fresh specimens contribute to increase the sensitivity in the diagnosis of trichomoniasis.

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Published

2016-01-01

Issue

Section

Original Articles

How to Cite

MENEZES, C. B., MELLO, M. dos S., & TASCA, T. (2016). COMPARISON OF PERMANENT STAINING METHODS FOR THE LABORATORY DIAGNOSIS OF TRICHOMONIASIS . Revista Do Instituto De Medicina Tropical De São Paulo, 58, e5-. https://www.revistas.usp.br/rimtsp/article/view/113122