THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL

Authors

  • Adriana Maria B. MENDONÇA Universidade Federal de Pernambuco
  • Ana Paula S. FEITOSA Universidade de Federal de Pernambuco; Department of Tropical Medicine
  • Dyana L. VERAS Universidade de Federal de Pernambuco; Department of Tropical Medicine
  • Thiago J. MATOS-ROCHA Universidade Federal de Pernambuco
  • Marília G. dos Santos CAVALCANTI Universidade de Federal da Paraíba; Department of Physiology and Pathology
  • Constança Clara G. S. BARBOSA Centro de Pesquisas Aggeu Magalhães; Department of Parasitology
  • Fábio A. BRAYNER Universidade de Federal de Pernambuco; Department of Tropical Medicine
  • Luiz C. ALVES Universidade Federal de Pernambuco

Abstract

Introduction: Schistosomiasis is a chronic disease caused by trematode flatworms of the genus Schistosoma and its control is dependent on a single drug, praziquantel (PZQ), but concerns over PZQ resistance have renewed interest in evaluating the in vitro susceptibility of recent isolates of Schistosoma mansoni to PZQ in comparison with well-established strains in the laboratory. Material and methods: The in vitro activity of PZQ (6.5-0.003 µg/mL) was evaluated in terms of mortality, reduced motor activity and ultrastructural alterations against S. mansoni. Results: After 3 h of incubation, PZQ, at 6.5 µg/mL, caused 100% mortality of all adult worms in the three types of recent isolates, while PZQ was inactive at concentrations of 0.08-0.003 µg/mL after 3 h of incubation. The results show that the SLM and Sotave isolates basically presented the same pattern of susceptibility, differing only in the concentration of 6.5 µg/mL, where deaths occurred from the range of 1.5 h in Sotave and just in the 3 h range of SLM. Additionally, this article presents ultrastructural evidence of rapid severe PZQ-induced surface membrane damage in S. mansoni after treatment with the drug, such as disintegration, sloughing, and erosion of the surface. Conclusion: According to these results, PZQ is very effective to induce tegument destruction of recent isolates of S. mansoni.

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Published

2016-01-01

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Original Articles

How to Cite

MENDONÇA, A. M. B., FEITOSA, A. P. S., VERAS, D. L., MATOS-ROCHA, T. J., CAVALCANTI, M. G. dos S., BARBOSA, C. C. G. S., BRAYNER, F. A., & ALVES, L. C. (2016). THE SUSCEPTIBILITY OF RECENT ISOLATES OF Schistosoma mansoni TO PRAZIQUANTEL . Revista Do Instituto De Medicina Tropical De São Paulo, 58, e7-. https://www.revistas.usp.br/rimtsp/article/view/113143