Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia

Authors

  • Diego H. Cáceres Corporación para Investigaciones Biológicas; Medical and Experimental Mycology Group
  • Alejandra Zuluaga Corporación para Investigaciones Biológicas; Medical and Experimental Mycology Group
  • Ángela M. Tabares Corporación para Investigaciones Biológicas; Medical and Experimental Mycology Group
  • Tom Chiller Centers for Disease Control and Prevention; Mycotic Diseases Branch
  • Ángel González Universidad de Antioquia; Escuela de Microbiología; Grupo de Investigación en Microbiología Básica y Aplicada
  • Beatriz L. Gómez Universidad del Rosario; Escuela de Medicina y Ciencias de la Salud

Keywords:

Cryptococcosis, Cryptococcus, Antigen, Diagnosis, Point-of-care, Lateral Flow Assay, Immunochromatographic assay, Latex agglutination system

Abstract

A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS®) and CrAg® LFA was observed. CrAg® LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen.

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Published

2017-01-01

Issue

Vol. 59 (2017)

Section

Brief Communication

How to Cite

Cáceres, D. H., Zuluaga, A., Tabares, Ángela M., Chiller, T., González, Ángel, & Gómez, B. L. (2017). Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia. Revista Do Instituto De Medicina Tropical De São Paulo, 59, e76. https://www.revistas.usp.br/rimtsp/article/view/143763