Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus

  • Maria Isabel de Oliveira Instituto Adolfo Lutz; Centro de Virologia; Núcleo de Doenças Respiratórias
  • Gislene Mitsue Namiyama Instituto Adolfo Lutz; Núcleo de Microscopia Eletrônica
  • Gabriela Bastos Cabral Instituto Adolfo Lutz; Centro de Virologia; Núcleo de Doenças de Vinculação Sexual e Sanguínea
  • João Leandro Ferreira Instituto Adolfo Lutz; Centro de Virologia; Núcleo de Doenças de Vinculação Sexual e Sanguínea
  • Noemi Taniwaki Instituto Adolfo Lutz; Núcleo de Microscopia Eletrônica
  • Ana Maria Sardinha Afonso Instituto Adolfo Lutz; Centro de Virologia; Núcleo de Doenças Respiratórias
  • Isabella Rillo Lima Instituto Adolfo Lutz; Centro de Virologia; Núcleo de Doenças Respiratórias
  • Luís Fernando Macedo de Brigido Instituto Adolfo Lutz; Centro de Virologia; Núcleo de Doenças de Vinculação Sexual e Sanguínea
Keywords: Zika virus, Rubella virus, Isolation, Genotype

Abstract

A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in acute phase of suspected rubella disease. The 50-year-old unvaccinated woman living in Sao Paulo, Brazil, was admitted to the emergency room with fever, headache, rash, arthralgia and prostration. Urine samples were collected for virus isolation and RT-qPCR. SIRC and Vero cells were inoculated with urine samples during 7 days. RT-qPCR was performed using measles virus (MV) and RV primers and both were found to be negative. After this result, RT-qPCR was performed for parvovirus B19, herpes virus 6 and ZIKV. The urine sample and the isolate were positive by Real Time PCR for ZIKV and negative for all other viruses tested. The sequences isolated are from the Asiatic lineage.

Downloads

Download data is not yet available.
Published
2018-01-01
How to Cite
Oliveira, M., Namiyama, G., Cabral, G., Ferreira, J., Taniwaki, N., Afonso, A., Lima, I., & Brigido, L. (2018). Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus. Revista Do Instituto De Medicina Tropical De São Paulo, 60, e15. Retrieved from https://www.revistas.usp.br/rimtsp/article/view/144543
Section
Case Report