Toxocara canis 30-35 kDa excretory-secretory antigen is an important marker in mice challenged by inocula containing different parasite load levels

Authors

  • Gabriela Rodrigues e Fonseca Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, LIM-06 - Imunopatologia da Esquistossomose, São Paulo, São Paulo, Brazil http://orcid.org/0000-0001-6047-3668
  • Marcelo Andreetta Corral Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, LIM-06 - Imunopatologia da Esquistossomose, São Paulo, São Paulo, Brazil http://orcid.org/0000-0003-2124-7021
  • Fabiana Martins de Paula Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, LIM-06 - Imunopatologia da Esquistossomose, São Paulo, São Paulo, Brazil Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, São Paulo, São Paulo, Brazil Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil http://orcid.org/0000-0003-3343-0149
  • Dirce Mary Correia Lima Meisel Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, LIM-06 - Imunopatologia da Esquistossomose, São Paulo, São Paulo, Brazil
  • Ronaldo Cesar Borges Gryschek Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, LIM-06 - Imunopatologia da Esquistossomose, São Paulo, São Paulo, Brazil Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, São Paulo, São Paulo, Brazil Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil http://orcid.org/0000-0002-8241-0884
  • Susana Angélica Zevallos Lescano Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, LIM-06 - Imunopatologia da Esquistossomose, São Paulo, São Paulo, Brazil Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil http://orcid.org/0000-0002-4263-690X

DOI:

https://doi.org/10.1590/S1678-9946202264009

Keywords:

BALB/c mice, Toxocara canis, Toxocariasis, Western blotting, Immunodiagnosis, Antigenic fractions

Abstract

The Western-blotting technique was applied to identify antigenic fractions of excretory-secretory Toxocara canis antigen recognized by IgG antibodies throughout an experimental infection in mice challenged by different inocula. Mice were inoculated with 5, 50 and 500 embryonated eggs and serum samples were collected 15, 30, 60, 90 and 120 days post-infection. Serum samples were analyzed using an excretory-secretory Toxocara antigen. Antibodies recognized antigenic fractions from 30 to 90 kDa. The protein fraction of 30-35 kDa was the most frequently recognized regardless of the size of inoculum and the stage of infection represented by the different collection times, but the antigenic recognition was more evident in groups infected with 50 and 500 eggs. This study presents an antigenic panel of the excretory-secretory antigen of T. canis and suggests that the 30-35 kDa antigenic fraction is a promising marker of the infection and should be further explored in future studies on experimental toxocariasis.

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Published

2022-05-16

How to Cite

Fonseca, G. R. e ., Corral, M. A. ., Paula, F. M. de ., Meisel, D. M. C. L. ., Gryschek, R. C. B. ., & Lescano, S. A. Z. . (2022). Toxocara canis 30-35 kDa excretory-secretory antigen is an important marker in mice challenged by inocula containing different parasite load levels. Revista Do Instituto De Medicina Tropical De São Paulo, 64, e09. https://doi.org/10.1590/S1678-9946202264009

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Original Article