Genetic variability of Leishmania (Leishmania) infantum causing human visceral leishmaniasis in the Southeastern Brazil

Authors

  • Vinicius Alves Lima Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Protozoologia (LIM-49), São Paulo, São Paulo, Brazil
  • Renata Elen Costa Silva Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil ; Universidade Federal de São Paulo, Laboratório de Endocrinologia Molecular e Translacional, São Paulo, São Paulo, Brazil
  • Luiz Henrique Moraes Caetano Camargo Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil
  • Roberto Mitsuyoshi Hiramoto Instituto Adolfo Lutz, São Paulo, São Paulo, Brazil
  • Elcio de Souza Leal Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, Pará, Brazil http://orcid.org/0000-0003-3463-0492
  • Lucia Maria Almeida Braz Universidade de São Paulo, Faculdade de Medicina, Departamento de Medicina Preventiva, São Paulo, São Paulo, Brazil http://orcid.org/0000-0002-6493-465X
  • José Angelo Lauletta Lindoso Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazi; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Protozoologia (LIM-49), São Paulo, São Paulo, Brazil; Instituto de Infectologia Emilio Ribas, São Paulo, São Paulo, Brazil http://orcid.org/0000-0002-4405-4401

DOI:

https://doi.org/10.1590/S1678-9946202365055

Keywords:

Leishmania infantum, Human visceral leishmaniasis, kDNA minicircle, Sequencing, Polymorphism

Abstract

Leishmania infantum is a protozoan that causes visceral leishmaniasis (VL) in the Americas and some regions of Europe. The disease is mainly characterized by hepatosplenomegaly and fever, and can be fatal. Factors related to the host and parasite can contribute to the transmission of Leishmania and the clinical outcome. The intraspecific genetic variability of L. infantum strains may be one of these factors. In this study, we evaluated the genetic variability of L. infantum obtained from bone marrow smear slides from patients in the Sao Paulo State, Brazil. For this, the minicircle of the kDNA hypervariable region was used as target by Sanger sequencing. By analyzing the similarity of the nucleotides and the maximum likelihood tree (Fasttree), we observed a high similarity (98%) among samples. Moreover, we identified four different profiles of L. infantum. In conclusion, L. infantum strains from Sao Paulo State, Brazil, showed low diversity measured by minicircle of the kDNA hypervariable region.

Downloads

Download data is not yet available.

Downloads

Published

2023-11-14

Issue

Vol. 65 (2023)

Section

Brief Communication

License

Copyright (c) 2023 Vinicius Alves Lima, Renata Elen Costa Silva, Luiz Henrique Moraes Caetano Camargo, Roberto Mitsuyoshi Hiramoto, Elcio de Souza Leal, Lucia Maria Almeida Braz, José Angelo Lauletta Lindoso

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Funding data

How to Cite

Lima, V. A. ., Silva, R. E. C. ., Camargo, L. H. M. C. ., Hiramoto, R. M. ., Leal, E. de S. ., Braz, L. M. A., & Lindoso, J. A. L. . (2023). Genetic variability of Leishmania (Leishmania) infantum causing human visceral leishmaniasis in the Southeastern Brazil. Revista Do Instituto De Medicina Tropical De São Paulo, 65, e55. https://doi.org/10.1590/S1678-9946202365055