Labeling of Salmonella Typhymurium with iodine-131 to study phagocytic function in rats

Authors

  • Maria K. Sato Universidade de São Paulo; Faculdade de Medicina; Departamento de Radiologia; Centro de Medicina Nuclear
  • Aldo J. Rodrigues Junior Universidade de São Paulo; Faculdade de Medicina
  • Edwaldo E. Camargo Universidade de São Paulo; Faculdade de Medicina; Departamento de Radiologia; Centro de Medicina Nuclear

Keywords:

Salmonella typhymurium, Iodine-131, Transplanted spleen

Abstract

The present study describes a method for labeling Salmonella typhymurium with iodine-131 to evaluate both the morphological and the functional characteristics of the reticulo-endothelial system. A suspension containing 2 x 10(9) bacteria per ml was labeled with carrier-free Na131I without reductor, with a labeling yield of 46.5 ± 3% and 3.5 ± 1.3% of free Iodine-131. The biodistribution of the labeled bacteria in rats was studied with a large field-of-view scintillation camera equiped with a pinhole collimator. Whole body images were obtained 15 and 30 minutes after intravenous injection of the labeled microorganisms. Images showed accumulation of bacteria in the liver and both normal and transplanted spleens of the animals. Autoradiographs of liver and spleen demonstrated labeled bacteria within the cells of the reticulo-endothelial system. The method described is easy to perform, has a good labeling yield and allows the functional evaluation of the reticulo-monophagocytic system, including transplanted spleens.

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Published

1989-06-01

Issue

Section

Original Articles

How to Cite

Sato, M. K., Rodrigues Junior, A. J., & Camargo, E. E. (1989). Labeling of Salmonella Typhymurium with iodine-131 to study phagocytic function in rats . Revista Do Instituto De Medicina Tropical De São Paulo, 31(3), 135-138. https://www.revistas.usp.br/rimtsp/article/view/28653