Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis

Authors

  • Aoi Masuda Instituto de Medicina Tropical de São Paulo; Laboratório de Sorologia e Imunologia
  • Sueli Fátima do Nascimento Instituto de Medicina Tropical de São Paulo; Laboratório de Sorologia e Imunologia
  • Carmem Silvia Guerra Instituto de Medicina Tropical de São Paulo; Laboratório de Sorologia e Imunologia
  • Gláucia da Silva Paranhos Instituto de Medicina Tropical de São Paulo; Laboratório de Sorologia e Imunologia
  • Antonio Walter Ferreira Instituto de Medicina Tropical de São Paulo; Laboratório de Sorologia e Imunologia

Keywords:

Antigens of Leishmania braziliensis braziliensis, Serodiagnosis of American cutaneous leishmaniasis

Abstract

The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b.braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease. The ACL sera reacted constantly with a 60 kD band. No reactivity to this protein was observed with sera from the other diseases above mentioned indicating that the 60 kD protein may be used in serodiagnosis for ACL.

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Published

1989-08-01

Issue

Vol. 31 No. 4 (1989)

Section

Original Articles

How to Cite

Masuda, A., Nascimento, S. F. do, Guerra, C. S., Paranhos, G. da S., & Ferreira, A. W. (1989). Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis . Revista Do Instituto De Medicina Tropical De São Paulo, 31(4), 228-234. https://www.revistas.usp.br/rimtsp/article/view/28668