Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA

Authors

  • Eide Dias Camargo Instituto Adolfo Lutz; Serology Section
  • Paulo Mutuko Nakamura Instituto Adolfo Lutz; Serology Section
  • Adelaide José Vaz Instituto Adolfo Lutz; Serology Section
  • Marcos Vinícius da Silva Emilio Ribas Hospital
  • Pedro Paulo Chieffi Instituto de Medicina Tropical
  • Elisabete Ourique de Melo Instituto de Medicina Tropical

Keywords:

Visceral larva migrans, Toxocara canis, A. suum absorption, immunodiagnosis of toxocariasis, dot-ELISA, ELISA

Abstract

The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of the two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.

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Published

1992-02-01

Issue

Vol. 34 No. 1 (1992)

Section

Serology

How to Cite

Camargo, E. D., Nakamura, P. M., Vaz, A. J., Silva, M. V. da, Chieffi, P. P., & Melo, E. O. de. (1992). Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA . Revista Do Instituto De Medicina Tropical De São Paulo, 34(1), 55-60. https://www.revistas.usp.br/rimtsp/article/view/28892