Schistosoma mansoni: identification of a 46KDa antigen of the schistosomular surface by monoclonal antibody

Authors

  • V. P. C. P. Toledo UFMG; Instituto de Ciências Biológicas; Departamento de Parasitologia
  • C. A. P. Tavares UFMG; Instituto de Ciências Biológicas; Departamento de Bioquímica e Imunologia
  • S. B. Henriques UFMG; Instituto de Ciências Biológicas; Departamento de Bioquímica e Imunologia
  • E. Nascimento UFMG; Instituto de Ciências Biológicas; Departamento de Parasitologia
  • M. Resende UFMG; Instituto de Ciências Biológicas; Departamento de Microbiologia

Keywords:

Schistosoma mansoni, Monoclonal antibody, Cercariae, Schistosomula

Abstract

An IgG2a subclass monoclonal antibody, C6G9, was obtained by immunization of BALB/c mice with Schistosoma mansoni egg antigens. With this monoclonal antibody, it was possible to identify a schistosomular antigen with a molecular weight of 46 kilodaltons (KDa), and its expression being evaluated by means of indirect immunofluorescence. The antigen persisted in the integument of the developing schistosomulum, for at least 96 hours post-transformation. The monoclonal antibody also reacted with the cercaria surface, but not with that of adult worm. The C6G9 was also able to mediate significant levels of cytotoxicity in the presence of complement for newly transformed schistosomula.

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Published

1994-06-01

Issue

Vol. 36 No. 3 (1994)

Section

Serology

How to Cite

Toledo, V. P. C. P., Tavares, C. A. P., Henriques, S. B., Nascimento, E., & Resende, M. (1994). Schistosoma mansoni: identification of a 46KDa antigen of the schistosomular surface by monoclonal antibody . Revista Do Instituto De Medicina Tropical De São Paulo, 36(3), 199-204. https://www.revistas.usp.br/rimtsp/article/view/29154