A simple PCR-based procedure for plaque diagnosis

Authors

  • Nilma Cintra Leal FIOCRUZ; Centra de Pesquisas Aggeu Magalhães
  • Frederico Guilherme Coutinho Abath FIOCRUZ; Centra de Pesquisas Aggeu Magalhães
  • Luis Carlos Alves FIOCRUZ; Centra de Pesquisas Aggeu Magalhães
  • Alzira Maria Paiva de Almeida FIOCRUZ; Centra de Pesquisas Aggeu Magalhães

Keywords:

Plague, Yersinia pestis, Rodents, PCR, Diagnosis

Abstract

Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals.

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Published

1996-10-01

Issue

Vol. 38 No. 5 (1996)

Section

Brief Communication

How to Cite

Leal, N. C., Abath, F. G. C., Alves, L. C., & Almeida, A. M. P. de. (1996). A simple PCR-based procedure for plaque diagnosis . Revista Do Instituto De Medicina Tropical De São Paulo, 38(5), 371-374. https://www.revistas.usp.br/rimtsp/article/view/29403