Evaluation of anti-Schistosoma mansoni igG antibodies in patients with chronic schistosomiasis mansoni before and after specific treatment
Keywords:
Schistosomiasis diagnosis, Schistosomiasis cure evaluation, Immunenzymatic tests, ImmunoblottingAbstract
The circumoval precipitin test (COPT), enzyme-linked immunosorbent assay (ELISA) and the immunoblotting anti-adult worm antigen (AWA) and soluble egg antigen (SEA) tests were applied to 17 chronically schistosome-infected patients for the detection of anti-Schistosoma mansoni antibodies before and on four occasions after oxamniquine administration over a period of six months. Compared to a control group, schistosomiasis patients showed high levels of IgG antibodies in AWA and SEA-ELISA. A decrease in IgG levels was observed six months after treatment, although negative reactions were not obtained. Significant decreases in IgG1, IgG3 and, mainly, IgG4, but not anti-SEA IgG2 levels were observed six months after treatment, again without negativity. Analysis of anti-AWA IgG antibodies by immunoblotting before treatment showed a 31 kDa strand in 14 patients (82%) which disappeared in three cases up to six months after treatment; furthermore, anti-SEA IgG antibodies showed the same band in nine patients (53%) before treatment, which disappeared in only four cases up to six months after treatment.Downloads
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Published
2001-06-01
Issue
Section
Schistosomiasis
How to Cite
VENDRAME, C. M. V., CARVALHO, M. D. T., YAMAMOTO, C. R. F., NAKHLE, M. C., CARVALHO, S. A., & CHIEFFI, P. P. (2001). Evaluation of anti-Schistosoma mansoni igG antibodies in patients with chronic schistosomiasis mansoni before and after specific treatment . Revista Do Instituto De Medicina Tropical De São Paulo, 43(3), 153-159. https://www.revistas.usp.br/rimtsp/article/view/30512