Molecular typing of dengue virus type 2 in Brazil

Authors

  • Marize P. Miagostovich Instituto Oswaldo Cruz; Departmento de Virologia; Laboratório de Flavivirus
  • Patrícia C. Sequeira Instituto Oswaldo Cruz; Departmento de Virologia; Laboratório de Flavivirus
  • Flávia B. dos Santos Instituto Oswaldo Cruz; Departmento de Virologia; Laboratório de Flavivirus
  • Amanda Maia Instituto Oswaldo Cruz; Departmento de Virologia; Laboratório de Flavivirus
  • Rita Maria R. Nogueira Instituto Oswaldo Cruz; Departmento de Virologia; Laboratório de Flavivirus
  • Hermann G. Schatzmayr Instituto Oswaldo Cruz; Departmento de Virologia; Laboratório de Flavivirus
  • Eva Harris University of California; School of Public Health; Division of Infectious Diseases
  • Lee W. Riley University of California; School of Public Health; Division of Infectious Diseases

Keywords:

Dengue virus type 2, Molecular epidemiology, Brazil

Abstract

Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype).

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Published

2003-01-01

Issue

Vol. 45 No. 1 (2003)

Section

Virology

How to Cite

Miagostovich, M. P., Sequeira, P. C., Santos, F. B. dos, Maia, A., Nogueira, R. M. R., Schatzmayr, H. G., Harris, E., & Riley, L. W. (2003). Molecular typing of dengue virus type 2 in Brazil . Revista Do Instituto De Medicina Tropical De São Paulo, 45(1), 17-21. https://www.revistas.usp.br/rimtsp/article/view/30664