Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania (Viannia) subgenus

Authors

  • Byanca Regina Paiva Universidade de São Paulo; Depto. de Parasitologia; Instituto de Ciências Biomédicas
  • Luciana Neves Passos Hospital Universitário Cassiano Antônio de Moraes; Ambulatório de Doenças Infecciosas e Parasitárias
  • Aloisio Falqueto Hospital Universitário Cassiano Antônio de Moraes; Ambulatório de Doenças Infecciosas e Parasitárias
  • Rosely dos S. Malafronte Universidade de São Paulo; Instituto de Medicina Tropical de São Paulo
  • Heitor Franco de Andrade Jr. Universidade de São Paulo; Depto. de Parasitologia; Instituto de Ciências Biomédicas

Keywords:

Leishmaniasis (Viannia), PCR, Identification

Abstract

In Brazil, the main etiologic agent of Leishmaniasis that frequently presents with mucosal involvement belongs to the Viannia subgenus. The therapeutic conduct in this disease depends on the parasitological diagnosis, and classical methods are restricted in identifying the agent. In this paper we describe a polymerase chain reaction (PCR), which uses primers designed from mini-exons repetitive sequences. The PCR amplifies a 177bp fragment that can distinguish (Viannia) from (Leishmania) subgenus. This test could be a useful diagnostic tool.

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Published

2004-12-04

Issue

Vol. 46 No. 6 (2004)

Section

Brief Communication

How to Cite

Paiva, B. R., Passos, L. N., Falqueto, A., Malafronte, R. dos S., & Andrade Jr., H. F. de. (2004). Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania (Viannia) subgenus . Revista Do Instituto De Medicina Tropical De São Paulo, 46(6), 335-338. https://www.revistas.usp.br/rimtsp/article/view/30859