Refractive index matching applied to fecal smear clearing
Keywords:
Turbidity of fecal suspensions, Thick fecal smears, Refractive index matchingAbstract
Thick smears of human feces can be made adequate for identification of helminth eggs by means of refractive index matching. Although this effect can be obtained by simply spreading a fleck of feces on a microscope slide, a glycerol solution has been routinely used to this end. Aiming at practicability, a new quantitative technique has been developed. To enhance both sharpness and contrast of the images, a sucrose solution (refractive index = 1.49) is used, which reduces the effect of light-scattering particulates. To each slide a template-measured (38.5 mm³) fecal sample is transferred. Thus, egg counts and sensitivity evaluations are easily made.Downloads
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Published
2005-12-01
Issue
Section
Brief Communication
How to Cite
Ferreira, C. S. (2005). Refractive index matching applied to fecal smear clearing . Revista Do Instituto De Medicina Tropical De São Paulo, 47(6), 347-350. https://www.revistas.usp.br/rimtsp/article/view/30953