Fecal specimens preparation methods for PCR diagnosis of human taeniosis

Authors

  • Cáris Maroni Nunes UNESP; FOA; Departamento de Apoio, Produção e Saúde Animal
  • Luiz Gustavo Ferraz Lima UNESP; FOA; Departamento de Apoio, Produção e Saúde Animal
  • Camila Santos Manoel UNESP; FOA; Departamento de Apoio, Produção e Saúde Animal
  • Rodrigo Norberto Pereira UNESP; FOA; Departamento de Apoio, Produção e Saúde Animal
  • Mauro Massaharu Nakano UNESP; FOA; Departamento de Apoio, Produção e Saúde Animal
  • José Fernando Garcia UNESP; FOA; Departamento de Apoio, Produção e Saúde Animal

Keywords:

Taenia saginata, DNA extraction, Polymerase chain reaction (PCR), Fecal specimens

Abstract

Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®.

Downloads

Download data is not yet available.

Downloads

Published

2006-02-01

Issue

Vol. 48 No. 1 (2006)

Section

Technical Report

How to Cite

Nunes, C. M., Lima, L. G. F., Manoel, C. S., Pereira, R. N., Nakano, M. M., & Garcia, J. F. (2006). Fecal specimens preparation methods for PCR diagnosis of human taeniosis . Revista Do Instituto De Medicina Tropical De São Paulo, 48(1), 45-47. https://www.revistas.usp.br/rimtsp/article/view/30968