Comparison of five methods for oxacillin susceptibility testing of Staphylococcus aureus isolates from cystic fibrosis patients

Authors

  • Marcelo J. Mimica Santa Casa School of Medicine; Department of Pediatrics; Division of Infectious Diseases
  • Rozane L. Carvalho Santa Casa School of Medicine; Department of Pathology; Division of Microbiology
  • Eitan N. Berezin Santa Casa School of Medicine; Department of Pediatrics; Division of Infectious Diseases
  • Neiva Damaceno Santa Casa School of Medicine; Department of Pediatrics; Division of Pulmonology
  • Hélio H. Caiaffa-Filho FMUSP; Hospital das Clinicas; Laboratory of Medical Investigation

Keywords:

Oxacillin, Cefoxitin, Staphylococcus aureus, mecA, Cystic fibrosis, MRSA

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) are now a worldwide problem. Cystic fibrosis (CF) patients are commonly colonized and infected by MRSA. Accurate oxacillin susceptibility testing is mandatory for the adequate management of these patients. We performed a comparison of the accuracy of different tests in CF isolates, including methicillin-susceptible S. aureus and MRSA with different SCCmec types, and using the mecA gene as the gold-standard. The sensitivity and specificity of oxacillin disc, Etest, and oxacillin agar screening plate were 100%. Sensitivity of the cefoxitin disc was 85% and specificity was 100%. For clinically relevant isolates, laboratories may consider the use of a combination of two phenotypic methods.

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Published

2012-12-01

Issue

Vol. 54 No. 6 (2012)

Section

Microbiology

How to Cite

Mimica, M. J., Carvalho, R. L., Berezin, E. N., Damaceno, N., & Caiaffa-Filho, H. H. (2012). Comparison of five methods for oxacillin susceptibility testing of Staphylococcus aureus isolates from cystic fibrosis patients. Revista Do Instituto De Medicina Tropical De São Paulo, 54(6), 305-306. https://www.revistas.usp.br/rimtsp/article/view/48421