Enterococcus faecium AND Enterococcus faecalis IN BLOOD OF NEWBORNS WITH SUSPECTED NOSOCOMIAL INFECTION

Authors

  • Isabela Furtado Federal University of Mato Grosso do Sul; Program Graduate Health and Development in the Midwest Region
  • Paula Cristhina Niz Xavier Federal University of Mato Grosso do Sul; Program Graduate Health and Development in the Midwest Region
  • Luciana Venhofen Martinelli Tavares Dom Bosco Catholic University and the Federal University of Mato Grosso do Sul; Department of Physical Therapy
  • Fabiana Alves Department of Physiology and Biofisica-ICB/UFMG
  • Sarah Fonseca Martins Department of Physiology and Biofisica-ICB/UFMG
  • Almir de Sousa Martins Department of Physiology and Biofisica-ICB/UFMG
  • Durval Batista Palhares Federal University of Mato Grosso do Sul; University Hospital; Department of Pediatrics

Abstract

Enterococci are Gram-positive cocci saprophyte of the human gastrointestinal tract, diners who act as opportunistic pathogens. They can cause infections in patients hospitalized for a long time or who have received multiple antibiotic therapy. Enterococcus faecalis and Enterococcus faecium are the most common species in human infections. To evaluate the possibility of rapid detection of these species and their occurrence in the blood of newborns with suspected nosocomial infection, blood samples were collected from 50 newborns with late infections, admitted to the Neonatal Care Unit of the University Hospital Federal de Mato Grosso do Sul (UFMS-HU), from September 2010 to January 2011. The samples were subjected to conventional PCR and real time PCR (qPCR) to search for Enterococcus faecium and Enterococcus faecalis, respectively. The PCR results were compared with respective blood cultures from 40 patients. No blood cultures were positive for Enterococci, however, eight blood samples were identified as genomic DNA of Enterococcus faecium by qPCR and 22 blood samples were detected as genomic DNA of Enterococcus faecalis by conventional PCR. These findings are important because of the clinical severity of the evaluated patients who were found positive by conventional PCR and not through routine microbiological methods.

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Published

2014-01-01

Issue

Section

Nosocomial Infections

How to Cite

Furtado, I., Xavier, P. C. N., Tavares, L. V. M., Alves, F., Martins, S. F., Martins, A. de S., & Palhares, D. B. (2014). Enterococcus faecium AND Enterococcus faecalis IN BLOOD OF NEWBORNS WITH SUSPECTED NOSOCOMIAL INFECTION . Revista Do Instituto De Medicina Tropical De São Paulo, 56(1), 77-80. https://www.revistas.usp.br/rimtsp/article/view/78701