Infectivity of amastigotes of Trypanosoma cruzi

  • Tecia Ulisses de Carvalho Universidade Federal do Rio de Janeiro; Instituto de Biofísica; Laboratório de Ultra-estrutura Celular
  • Wanderley de Souza Universidade Federal do Rio de Janeiro; Instituto de Biofísica; Laboratório de Ultra-estrutura Celular

Abstract

The infectivity amastigotes of Trypanosoma cruzi, isolated from the supernatant of the J774G8 macrophage-like cell line infected with trypomastigotes to normal macrophages in vitro was tested. After a period of 1 h of T. cruzi-macrophage interaction about 2% of the mouse peritoneal macrophages had ingested amastigotes. In contrast 12% of the macrophages had ingested epimastigotes. Treatment of the amastigotes with trypsin did not interfere with their ingestion by macrophages. Once inside the macrophages the amastigotes divided and after some days transformed into trypomastigotes. When i.p. inoculated into mice the amastigotes were highly infective, inducing high levels of parasitaemia and tissue parasitism. As previously described for trypomastigotes, amastigotes were not lysed when incubated in the presence of fresh guinea-pig serum. Contrasting with what has been described for trypomastigotes, the resistance of amastigotes to complement-mediated lysis persisted after treatment with trypsin.

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Published
1986-08-01
How to Cite
Carvalho, T., & Souza, W. (1986). Infectivity of amastigotes of Trypanosoma cruzi . Revista Do Instituto De Medicina Tropical De São Paulo, 28(4), 205-212. Retrieved from https://www.revistas.usp.br/rimtsp/article/view/87490
Section
Original Articles