Perfil microbiológico da colonização do sítio de inserção do cateter venoso central considerando dois curativos transparentes

Autores

DOI:

https://doi.org/10.11606/issn.2176-7262.v53i2p135-145

Palavras-chave:

Cateteres Venosos Centrais, Bandagens, Curativos Oclusivos, Microbiologia

Resumo

Modelo do Estudo: estudo transversal da fase prospectiva de um ensaio clínico. Objetivo: identificar os microrganismos e a sensibilidade antimicrobiana no sítio de inserção do cateter venoso central coberto pelo curativo gel de clorexidina ou pelo filme transparente de poliuretano. Metodologia: estudo transversal, descritivo, realizado com adultos críticos no período de abril a dezembro de 2014 em um hospital universitário no interior do estado de São Paulo. Imediatamente após a retirada do curativo, foi coletada amostra de swab da pele do sítio de inserção do cateter, semeado em placas de Agar sangue e Agar MacConkey e incubadas em estufas bacteriológicas a 35 °C. Nas amostras que apresentaram crescimento bacteriano após 24 horas de incubação, foram realizados testes de sensibilidade aos antimicrobianos utilizando o equipamento automatizado Vitek II (Biomerieux®). Resultados: 45 pacientes fizeram uso do curativo gel de clorexidina e 47 utilizaram o filme transparente de poliuretano. No grupo com o curativo gel de clorexidina houve crescimento dos microrganismos Acinetobacter baumannii, Pseudomonas aeruginosa, Morganella morganii, Enterobacter cloacae, Staphylococcus aureus e Staphylococcus epidermidis em 13 amostras de swabs. O Staphylococcus aureus apresentou resistência a oxacilina. No grupo do filme transparente de poliuretano seis amostras foram positivas com o crescimento de Serratia marcescens, Acinetobacter baumannii, Staphylococcus epidermidis, Staphylococcus haemolyticus e Klebsiella pneumoniae, esta resistente a amicacina (Klebsiella pneumoniae carbapenemase - KPC). Conclusão: os resultados demonstram maior crescimento bacteriano no sítio de inserção do cateter venoso central coberto pelo curativo gel de clorexidina quando comparado ao filme transparente de poliuretano.

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Referências

Centers for Diseases Control and Prevention (CDC). Bloodstream infection event (central line-associated bloodstream infection and non-central line-associated bloodstream infection) [Internet]. Atlanta: Centers for Diseases Control and Prevention; 2020 [updated 2020 Jan]. Available from: https://www.cdc.gov/nhsn/pdfs/pscmanual/4psc_clabscurrent.pdf

O'Grady NP, Alexander M, Burns LA, Dellinger EP, Garland J, Heard SO, et al. Guidelines for the prevention of intravascular catheter-related infections. Am J Infect Control 2011; 39(4 Suppl 1):S1-34. https://doi.org/10.1093/cid/cir257

Webster J, Gillies D, O'Riordan E, Sherriff KL, Rickard CM. Withdrawn: gauze and tape and transparent polyurethane dressings for central venous catheters. Cochrane Database Syst Rev 2016; (5):CD003827. https://doi.org/10.1002/14651858.CD003827

Ullman AJ, Cooke ML, Mitchell M, Lin F, New K, Long DA, et al. Dressings and securement devices for central venous catheters (CVC). Cochrane Database Syst Rev 2015; (9):CD010367. https://doi.org/10.1002/14651858.CD010367.pub2

Bashir MH, Olson LK, Walters SA. Suppression of regrowth of normal skin flora under chlorhexidine gluconate dressings applied to chlorhexidine gluconate-prepped skin. Am J Infect Control 2012;40(4):344-8. https://doi.org/10.1016/j.ajic.2011.03.030

Jeanes A, Bitmead J. Reducing bloodstream infection with a chlorhexidine gel IV dressing. Br J Nurs 2015; 24(19): S14-9. https://doi.org/10.12968/bjon.2015.24.Sup19.S14

Safdar N, O'Horo JC, Ghufran A, Bearden A, Didier ME, Chateau D, et al. Chlorhexidine-impregnated dressing for prevention of catheter-related bloodstream infection: a meta-analysis. Crit Care Med 2014; 42(7):1703-13. https://doi.org/10.1097/CCM.0000000000000319

Margatho AS, Ciol MA, Hoffman JM, Reis PED, Furuya RK, Lima DAFS, et al. Chlorhexidine-impregnated gel dressing compared with transparent polyurethane dressing in the prevention of catheter-related infections in critically ill adult patients: A pilot randomised controlled trial. Aust Crit Care 2019;471-478. https://doi.org/10.1016/j.aucc.2018.11.001

Kawamura H, Takahashi N, Takahashi M, Taketomi A. Differences in microorganism growth on various dressings used to cover injection sites: inspection of the risk of catheter-related bloodstream infections caused by Gram-negative bacilli. Surg Today 2014; 44(12):2339-44. https://doi.org/10.1007/s00595-014-0935-z

Choudhury MA, Sidjabat HE, Rathnayake IU, Gavin N, Chan RJ, Marsh N, et al. Culture-independent detection of chlorhexidine resistance genes qacA/B and smr in bacterial DNA recovered from body sites treated with chlorhexidine-containing dressings. J Med Microbiol 2017; 66(4):447-53. https://doi.org/10.1099/jmm.0.000463

Carty N, Wibaux A, Ward C, Paulson DS, Johnson P. Antimicrobial activity of a novel adhesive containing chlorhexidine gluconate (CHG) against the resident microflora in human volunteers. J Antimicrob Chemother 2014; 69(8):2224-9. https://doi.org/10.1093/jac/dku096

Carrer S, Bocchi A, Bortolotti M, Braga N, Gilli G, Candini M, et al. Effect of different sterile barrier precautions and central venous catheter dressing on the skin colonization around the insertion site. Minerva Anestesiol 2005;71(5):197-206. https://www.minervamedica.it/en/journals/minerva-anestesiologica/article.php?cod=R02Y2005N05A0197

Gerçeker GÖ, Yardımcı F, Aydınok Y. Randomized controlled trial of care bundles with chlorhexidine dressing and advanced dressings to prevent catheter-related bloodstream infections in pediatric hematology-oncology patients. Eur J Oncol Nurs 2017; 28:14-20. https://doi.org/10.1016/j.ejon.2017.02.008

Biehl LM, Huth A, Panse J, Kramer C, Hentrich M, Engelhardt M, et al. A randomized trial on chlorhexidine dressings for the prevention of catheter-related bloodstream infections in neutropenic patients. Ann Oncol 2016;27(10):1916–22. https://doi.org/10.1093/annonc/mdw275

Düzkaya DS, Sahiner NC, Uysal G, Yakut T, Çitak A. Chlorhexidine-impregnated dressings and prevention of catheter-associated blood- stream infections in a pediatric intensive care unit. Crit Care Nurse 2016;36(6): e1-e7. https://doi.org/10.4037/ccn2016561

Wang HX, Xie SY, Wang H, Chu HK. The effects of chlorhexidine dressing on health care-associated infection in hospitalized patients: a meta-analysis. Iran J Public Health 2019; 48(5):796-807. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717407/

Eggimann P, Pagani JL, Dupuis-Lozeron E, Ms BE, Thévenin MJ, Joseph C, et al. Sustained reduction of catheter-associated bloodstream infections with enhancement of catheter bundle by chlorhexidine dressings over 11 years. Intensive Care Med 2019;45(6):823-33. https://doi.org/10.1007/s00134-019-05617-x

Timsit JF, Schwebel C, Bouadma L, Geffroy A, Garrouste-Orgeas M, Pease S, et al. Chlorhexidine impregnated sponges and less frequent dressing changes for prevention of catheter-related infections in critically ill adults: a randomized controlled trial. Jama 2009;301(12):1231–41.https://doi.org/10.1001/jama.2009.376

Timsit JF, Mimoz O, Mourvillier B, Souweine B, Garrouste-Orgeas M, Alfandari S, et al. Randomized controlled trial of chlorhexidine dressing and highly adhesive dressing for preventing catheter-related infections in critically ill adults. Am J Respir Crit Care Med 2012;186(12):1272–8. https://doi.org/10.1164/rccm.201206-1038OC

Wei L, Li Y, Li X, Bian L, Wen Z, Li M. Chlorhexidine-impregnated dressing for the prophylaxis of central venous catheter related complications: a systematic review and meta-analysis. BMC Infect Dis 2019;19(1):429. https://doi.org/10.1186/s12879-019-4029-9

Marschall J, Mermel LA, Fakih M, Hadaway L, Kallen A, O'Grady NP, et al. Strategies to prevent central line-associated bloodstream infections in acute care hospitals: 2014 update. Infect Control Hosp Epidemiol 2014;35(Suppl 2):S89-107.

Koljalg S, Naaber P, Mikelsaar M. Antibiotic resistance as an indicator of bacterial chlorhexidine susceptibility. J Hosp Infect 2002; 51(2):106-13. https://doi.org/10.1053/jhin.2002.1204

Lima MFP, Borges MA, Parente RS, Victória Júnior RC, Oliveira ME. Staphylococcus aureus e as infecções hospitalares – revisão de literatura. UNINGÁ Rev 2015;21(1):32-9. http://revista.uninga.br/index.php/uningareviews/article/view/1616>.

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2020-08-07

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1.
Pereira GL, Margatho AS, Lima DAF dos S, Santos BN, Reis PED dos, Basile-Filho A, et al. Perfil microbiológico da colonização do sítio de inserção do cateter venoso central considerando dois curativos transparentes. Medicina (Ribeirão Preto) [Internet]. 7º de agosto de 2020 [citado 16º de abril de 2024];53(2):135-4. Disponível em: https://www.revistas.usp.br/rmrp/article/view/163452