Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses

  • Gabriella Dias Arena Agronomic Institute/Sylvio Moreira Citrus Research Center
  • Pedro Luis Ramos-González Biological Institute
  • Maria Andréia Nunes Agronomic Institute/Sylvio Moreira Citrus Research Center
  • Camila Chabi Jesus Biological Institute
  • Renata Faier Calegario Federal University of Paraná
  • Elliot Watanabe Kitajima University of São Paulo/ESALQ; Dept. of Plant Pathology and Nematology
  • Valdenice Moreira Novelli Agronomic Institute/Sylvio Moreira Citrus Research Center
  • Juliana Freitas-Astúa Biological Institute
Keywords: Clerodendrum chlorotic spot virus, Cilevirus, Solanum violaefolium ringspot virus, Coffee ringspot virus, Dichorhavirus

Abstract

Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV.

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Published
2017-02-01
How to Cite
Arena, G., Ramos-González, P., Nunes, M., Jesus, C., Calegario, R., Kitajima, E., Novelli, V., & Freitas-Astúa, J. (2017). Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses. Scientia Agricola, 74(1), 85-89. https://doi.org/10.1590/1678-992x-2015-0380
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