Salt tolerance in bean (Paseolus vulgaris) cell culture
DOI:
https://doi.org/10.1590/S0103-90161995000100028Keywords:
plant tissue culture, proline content, salt tolerance, peroxidase activityAbstract
One of the applications of the tissue culture technique in plant improvement is the identification of cell lines which show salinity tolerance. Several authors were able to obtain saline stress-tolerant cell lines and show that mechanisms of tolerance to salts have a strong correlation between this phenomenon and a high macromolecule concentration in plant tissues. Callus obtained from embrionic axis of Phaseolus vulgarís cv. IAC carioca in solid medium, supplemented with 0 to 60 mM NaCl, as the salt treatment, were used. Callus harvesting was done on the 13th day, when they were processed for relative growth, protein, proline content and peroxidase acivity. The results show both, a decrease of the relative growth and of protein content in response to the NaCl treatment, as compared to controls. However, there was a significant increase on the proline content and on the peroxidase activity.Downloads
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Published
1995-04-01
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All content of the journal, except where identified, is licensed under a Creative Common attribution-type BY-NC.How to Cite
Salt tolerance in bean (Paseolus vulgaris) cell culture . (1995). Scientia Agricola, 52(1), 164-166. https://doi.org/10.1590/S0103-90161995000100028