Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies

Authors

  • Ildon Rodrigues do Nascimento UFT
  • Wilson Roberto Maluf UFLA; Depto. de Fitotecnia
  • Antônia Reis Figueira UNICENTRO; Depto. de Agronomia
  • Cícero Beserra Menezes UFLA; Depto. de Fitopatologia
  • Juliano Tadeu Vilela de Resende UFT
  • Marcos Ventura Faria UFT
  • Douglas Willian Nogueira UFLA; Depto. de Fitotecnia

DOI:

https://doi.org/10.1590/S0103-90162009000300003

Keywords:

Lycopersicon esculentum, Sw-5, vírus resistance, molecular marker

Abstract

The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.), which confers resistance to the tomato spotted wilt virus (TSWV). However, it had not been tested yet in advanced tomato populations. The goal of this study was to distinguish resistant homozygotes (Sw-5/Sw-5) and heterozygotes (Sw-5/Sw-5+) from susceptible (Sw-5+/Sw-5+) plants in crossing populations with the Stevens cultivar and advanced backcrossing populations by using 'Sw421' SCAR marker. The amplification of 940 bp and 900 bp bands characterized the resistant homozygotes and susceptible controls, respectively. A two band pattern (900 bp and 940 bp) was observed in heterozygote genotypes (Sw-5/Sw-5+), which confirmed the co-dominant inheritance mechanism of the marker. Fifty seven plants from the isogenic progenies were characterized based on bands pattern: 18 plants (31.6%) were identified as resistant homozygotes, 8 plants (14.0%) as resistant heterozygotes and 31 plants (54.4%) were characterized as susceptible. The SCAR 'Sw-421' marker is an important tool for selection and pyramid resistance alleles, mainly when other resistance sources to the TSWV are available, such as the Rey de los Tempranos source.

Downloads

Download data is not yet available.

Downloads

Published

2009-06-01

How to Cite

Nascimento, I. R. do, Maluf, W. R., Figueira, A. R., Menezes, C. B., Resende, J. T. V. de, Faria, M. V., & Nogueira, D. W. (2009). Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies . Scientia Agricola, 66(3), 298-303. https://doi.org/10.1590/S0103-90162009000300003

Issue

Section

Genetics and Plant Breeding