ESTs analysis in maize developing kernels exposed to single and combined water and heat stresses

Abstract

Molecular and metabolic response of plants to a combination of two abiotic stresses is unique and cannot be directly extrapolated from the response of plants to each of the stresses individually. cDNA macroarray has become a useful tool to analyze expression profiles and compare the similarities and differences of various expression patterns. A macroarray of approximately 2,500 maize (Zea mays L.) cDNAs was used for transcriptome profiling in response to single and simultaneous application of water and high temperature stress of maize developing kernels at 15 days after pollination. All stress treatments (water stress-WS, heat stress-HS and their combined application-CS) induced changes in expression of 106 transcripts with 54 up-regulated and 52 down-regulated. There were 11 up-regulated and 15 down-regulated transcripts in common for all three stresses. Although these common transcripts showed existence of a mutual mechanism in stress response, the 23 transcripts induced only in CS indicate that plants responded in a different manner when exposed to simultaneous effects of both stresses. A glimpse of functions regulated under WS, HS and CS is provided, and also the common and different responses between individual and simultaneous stresses.