Validation of spectrophotometric method to detect and quantify nitrite in ham pate

Authors

  • Patrícia Barros Reis Federal University of Minas Gerais; School of Veterinary Medicine; Departament of Technology and Inspection of Products from Animal Origin
  • Rodrigo Mauro Ramos Livestock and Supplying Ministry; National Agricultural Laboratory
  • Leonardo Francisco de Souza Livestock and Supplying Ministry; National Agricultural Laboratory
  • Silvana de Vasconcelos Cançado Federal University of Minas Gerais; School of Veterinary Medicine; Departament of Technology and Inspection of Products from Animal Origin

DOI:

https://doi.org/10.1590/S1984-82502009000100006

Keywords:

Validation, Nitrite, Spectrophotometric method, Ham pate

Abstract

The objective of this work was to validate the spectrophotometric method to detect and quantify nitrite using ham pate as a source. The validated analytical conditions were 540 nm wavelength and the samples reading between 40 to 70 minutes after addition of coloring agents. The tested performance criteria were: linearity, matrix effect, selectivity, detection and quantification limit, accuracy, precision and robustness. As the results have obtained linearity in the studied zone (0.125 to 3 g/mL) and matrix effect was not observed, there was not any interference from ascorbic acid, but interference occurred from sodium eritorbate, in concentrations above 2.5 mg/100g. The detection limit was defined as 12.5 mg of nitrite/kg for pate and the quantification limit as 25 mg of nitrite/kg of pate. The method presented repeatability and reproducibility. The accuracy was assessed through recovery values varying from 84 to 110%. Respecting robustness, it was observed that the variations applied to the extraction procedure depended on the concentration of nitrite in the pate sample. In conclusion, the methodology was validated under the tested conditions.

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Published

2009-03-01

Issue

Section

Original Papers

How to Cite

Validation of spectrophotometric method to detect and quantify nitrite in ham pate . (2009). Brazilian Journal of Pharmaceutical Sciences, 45(1), 49-55. https://doi.org/10.1590/S1984-82502009000100006