Application of redox reactions for the determination of valganciclovir hydrochloride in pharmaceuticals

Authors

  • Nagib Abdullah Salem Qarah University of Mysore, Department of Chemistry
  • Kanakapura Basavaiah University of Mysore, Department of Chemistry https://orcid.org/0000-0002-5447-0628
  • Sameer Abdulaziz Mohammed Abdulrahman Rada’a, Al-Baydha University, Faculty of Education and Sciences, Department of Chemistry

DOI:

https://doi.org/10.1590/s2175-97902019000317784

Keywords:

Valganciclovir hydrochloride/determination, Spectrophotometry/method validation, Pharmaceutical formulations

Abstract

Two simple, selective and sensitive spectrophotometric methods were developed and validated for the determination of valganciclovir hydrochloride (VLGH) in pure drug and tablets. The first method was based on the reduction of iron(III) to iron(II) by VLGH and subsequent formation of iron(III)-ferricyanide complex (Prussian blue) in acid medium which was measured at 730 nm (method A). In the second method (method B), permanganate was reduced by VLGH to bluish green manganate in alkaline medium and the absorbance was measured at 610 nm. The absorbance measured in each case was related to VLGH concentration. The experimental conditions were carefully studied and optimized. Beer’s law was obeyed over the concentration ranges of 2.5-20.0 and 2.0-40.0 µg mL-1 for method A and method B, respectively, with corresponding molar absorptivity values of 1.28×104 and 6.88×103 L mol-1 cm-1. The limits of detection (LOD) and quantification (LOQ) were 0.11 and 0.33 µg mL-1 (method A) and 0.21 and 0.64 µg mL-1 (method B). Within-day and between-day relative standard deviations (%RSD) at three different concentrations levels were < 2.4%, and the respective relative errors (%RE) were ≤ 3%. The proposed methods were successfully applied to the determination of VLGH in tablets, and the results confirmed that the proposed methods were equally precise and accurate as the official method.

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Published

2020-12-09

How to Cite

Qarah, N. A. S. ., Basavaiah, K. ., & Abdulrahman, S. A. M. . (2020). Application of redox reactions for the determination of valganciclovir hydrochloride in pharmaceuticals. Brazilian Journal of Pharmaceutical Sciences, 56, e17784. https://doi.org/10.1590/s2175-97902019000317784

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