Quantitative analysis of phenobarbital in biological fluids: Analyte enrichment by an electrically-assisted microextraction technique

Authors

  • Saeid Yaripour Tehran University of Medical Sciences, Faculty of Pharmacy, Department of Drug and Food Control https://orcid.org/0000-0002-5310-938X
  • Shahriar Ebrahimi Tehran University of Medical Sciences, Faculty of Pharmacy, Department of Drug and Food Control
  • Ali Mohammadi Tehran University of Medical Sciences, Faculty of Pharmacy, Department of Drug and Food Control

DOI:

https://doi.org/10.1590/s2175-97902019000417839%20A

Keywords:

Biological samples, Electromembrane extraction, HPLC-UV, Microextraction, Phenobarbital

Abstract

An electrically-assisted microextraction method called electromembrane extraction, followed by a simple high performance liquid chromatography and ultraviolet detection was developed and validated for determining phenobarbital in biological samples. The major parameters influencing the electromembrane extraction procedure including solvent composition, voltage, pH of acceptor and donor solutions, salt effect, and time of extraction were evaluated and optimized. The drug was extracted from the donor aqueous sample solution (pH 9) to the acceptor aqueous solution (pH 13). The donor and acceptor phases were separated by a hollow fiber dipped in 1-octanol as a supported liquid membrane. A voltage of 40 V during 20 minutes was applied as the driving force. The enrichment factor was obtained >51 which enhanced the sensitivity of the instrument. Limit of detection and limit of quantitation were 7.5 and 25 ng/ mL, respectively. The method was linear over the range of 25-1000 ng/mL for phenobarbital (R2 >0.9998) with repeatability (%RSD) between 0.4% and 6.8% (n = 3). The proposed method was successfully applied to human plasma and urine samples with relative recovery of 70-80% and %RSD < 6.8%.

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Published

2020-12-09

How to Cite

Yaripour, S. ., Ebrahimi, S. ., & Mohammadi, A. . (2020). Quantitative analysis of phenobarbital in biological fluids: Analyte enrichment by an electrically-assisted microextraction technique. Brazilian Journal of Pharmaceutical Sciences, 56, e17839. https://doi.org/10.1590/s2175-97902019000417839 A

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