Nanostructured lipid carriers as a novel tool to deliver sclareol: physicochemical characterisation and evaluation in human cancer cell lines

Gabriel Silva Marques Borges; Pedro  Henrique Dias Moura Prazeres; Ângelo  Malachias de Souza; Maria Irene Yoshida; José Mario Carneiro Vilela; Aline Teixeira Maciel e Silva; Mariana Silva Oliveira; Dawidson Assis Gomes; Margareth Spangler Andrade; Elaine Maria  de Souza-Fagundes; Lucas Antônio Miranda Ferreira

doi:10.1590/s2175-97902020000418497

Authors

Gabriel Silva Marques Borges Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil https://orcid.org/0000-0002-7360-8231
Pedro Henrique Dias Moura Prazeres Department of Physiology and Biophysics, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Brazil https://orcid.org/0000-0002-1933-3230
Ângelo Malachias de Souza Department of Physics, Institute of Exact Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
Maria Irene Yoshida 4 Department of Chemistry, Institute of Exact Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil,
José Mario Carneiro Vilela Technology and Innovation Center, Industrial Learning National Service, Belo Horizonte, Brazil
Aline Teixeira Maciel e Silva Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazi
Mariana Silva Oliveira Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil
Dawidson Assis Gomes Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Brazil
Margareth Spangler Andrade Technology and Innovation Center, Industrial Learning National Service, Belo Horizonte, Brazil
Elaine Maria de Souza-Fagundes Department of Physiology and Biophysics, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Brazil,
Lucas Antônio Miranda Ferreira https://orcid.org/0000-0003-2474-5536

DOI:

https://doi.org/10.1590/s2175-97902020000418497

Keywords:

Cancer. Sclareol. Solid lipid nanoparticles. Nanostructured lipid carriers. Small-angle X-ray scattering.

Abstract

Sclareol (SC) is arousing great interest due to its cytostatic and cytotoxic activities in several cancer cell lines. However, its hydrophobicity is a limiting factor for its in vivo administration. One way to solve this problem is through nanoencapsulation. Therefore, solid lipid nanoparticles (SLN-SC) and nanostructured lipid carriers (NLC-SC) loaded with SC were produced and compared regarding their physicochemical properties. NLC-SC showed better SC encapsulation than SLN-SC and was chosen to be compared with free SC in human cancer cell lines (MDA-MB-231 and HCT-116). Free SC had slightly higher cytotoxicity than NLC-SC and produced subdiploid DNA content in both cell lines. On the other hand, NLC-SC led to subdiploid content in MDA-MB-231 cells and G2/M checkpoint arrest in HCT-116 cells. These findings suggest that SC encapsulation in NLC is a way to allow the in vivo administration of SC and might alter its biological properties.

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Nanostructured lipid carriers as a novel tool to deliver sclareol

physicochemical characterisation and evaluation in human cancer cell lines

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