Characterization and identification of ascorbyl methylsilanol pectinate for cosmetic formulations application
DOI:
https://doi.org/10.1590/s2175-97902020000419023Keywords:
Derivative of ascorbic acid. HPLC analysis. Antioxidant activity.Abstract
The ascorbyl methylsilanol pectinate (AMP) presents the same functional properties of ascorbic acid (AA). Besides antioxidant and depigmentant activity, the AMP presents silanol in its chemical structure. The aim of this work was to characterize and indentify the AMP alone and in cosmetic formulations. The following techniques were employed: Fourier Transform Infrared Spectrophotometry, particle size distributions, in vitro antioxidant activity with 2.2-diphenyl-1 picrylhydrazyl (DPPH) and Oxigen Radical Absorbance Capacity Assay and High Performace Liquid Chromatography (HPLC) (developed and validated method) for the active ingredient; Microscopy, HPLC and Normal Stability Assay (NSA) for the emulsions. Particle size distributions results showed that the average size of AMP was 1.0 µm and polydispersity index was 0.1. In DPPH assay AA and AMP were statistically the same. The value of ORAC obtained for AMP was 0.74 and for AA in the literature was 0.95. In the NSA the formulations were stable in conditions of 5.0 and 45.0 ± 2.0 ºC for 90 days. Adequate stability at ambient temperature out of reach of light was also observed. Thus, this works presented an acceptable method for quantification of AMP alone and in cosmetic formulations. AMP was an adequate choice for the incorporation in emulsions with antioxidant efficacy.
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Aguilera JD, Gálvez MV, Sánchez C, Herrera-Ceballos E. Changes in photoinduced cutaneous erythema with topical application of a combination of vitamins C and E before and after UV exposure. J Dermatol Sci. 2012;66(3):216-20.
Anton N, Vandamme TF. The universality of low-energy nano-emulsification. Int J Pharm. 2009;377:142-147.
Baby A.R. Avaliação in vitro da permeabilidade cutânea da rutina em emulsões cosméticas. São Paulo, 2007. 170p. Tese de Doutorado - Faculdade de Ciências Farmacêuticas - Universidade de São Paulo.
Burke-colvi D, Hines M, Gan D. Skin care formulations, 8/048/456 (2001).
Brazil. Ministry of Health. National Sanitary Surveillance Agency. Guide to the stability of cosmetic products. Brasília: ANVISA Press; 2004. 45p.
Clauss F, Baeza R. Talc substances having specific Surface properties, methods of Manufacture and applications, 5/229/094, (1993).
Dario MF, Santos MSCS, Viana AS, Arêas EPG, Bou-Chacra NA, Oliveira MC, et al. A high loaded cationic nanoemulsion for quercetin delivery obtained by sub-PIT method. Colloids Surfaces A Physicochem Eng Asp. 2016;489:256-264.
Exsymol. Ascorbosilane C. Technical Document. Monaco, 5p, 1998.
Gonçalves SMF. Vitamina C na cosmecêutica. Revista Racine. 2001;64:22-29.
Gaspar LR, Campos PMBGM. Photostability and efficacy studies of topical formulations containing UV-filters combination and vitamins A, C and E. Int J Pharm. 2007;343:181-9.
Halliwell B, Gutteridge JMC. (1999) Free Radicals in Biology and Medicine, 3rd edn. Oxford: Clarendon Press, 1-25p.
ICH, ICH Q2B - International Conference on Harmonization. Q2 B Validation of Analytical Procedures: Methodology, (1996) section VIII. http://www.fda.gov/downloads/drugs/guidancecomplianceregulatoryinformation/guidances/ucm073384.pdf (Accessed in 29 november 2016).
» http://www.fda.gov/downloads/drugs/guidancecomplianceregulatoryinformation/guidances/ucm073384.pdf
Kaszuba M. Dynamic Light Scattering. Malvern Guid. 2015;1-26.
Kim S, Lee TG. Stabilization of L-ascorbic acid in cosmetic emulsions. J Ind Eng Chem. 2018;57:193-198.
Kundu P, Brenowitz ND, Voon V, Worbe Y, Vertes PE, Inati SJ et al. An Integrated strategy for improving functional connectivity mapping using multi-echo EPI. Proc Natl Acad Sci. 2013;110(40):1-7.
López-alarcón C, Lissi E.A novel and simple ORAC methodology based on the interaction of Pyrogallol Red with peroxyl radicals. Free Radical Research. 2006;40(9):979-985.
Maia AM. Desenvolvimento e avaliação da estabilidade de formulações cosméticas contendoácido ascórbico. dissertação . São Paulo:Universidade de São Paulo, Faculdade de Ciências Farmacêuticas; 2002.117p.
Murdock RC, Braydich-Stolle L, Schrand AM, Schlager JJ. Hussains M. Characterization of Nanomaterial Dispersion in Solution Prior to In Vitro Exposure Using Dynamic Light Scattering Technique. Toxicol Sci. 2008;101(2),239-253.
Oliveira CA, Peres DDA, Graziola F, Bou-Chacra NA, Araujo GLB, Flórido AC, Mota J, Rosado C, Velasco MVR, Rodrigues LM, Fernandes AF, Baby AR. Cutaneous biocompatible rutin-loaded gelatin-based nanoparticles increase the SPF of the association of UVA and UVB filter. Eur J Pharm Sci. 2016;81:1-9.
Ou B, Woodill-hampsch M, Prior RL. Development and Validation of Improved Oxygen Radical Absorbance Capacity Assay Using Fluorecein as the Fluorescent Probe. J Agric Food Chem. 2001;49:4619-4626.
Prista LN, Alves AC, Morgado R. Tecnologia farmacêutica. 5.ed. Lisboa:Fundação Calouste Gulbenkian, 1995. P. 597-668.
Santos ODH. Desenvolvimento e avaliação das propriedades físico-químicas e atividade cosmética in vivo de emulsões de óleo de Calendula officinalis com cristal líquido. Ribeirão Preto, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Doutorado, 133p, 2006.
Sharma OP, Bhat TK. DPPH antioxidant assay revisited. Analytical Methods. 2009;113:1202-1205.
Sheraz MA, Khan MF, Ahmed S, Kazi SH, Ahmed I. Stability and Stabilization of Ascorbic Acid. Household Pers Care Today. 2015;10(3):22-25.
Srilatha R, Apharna C, Srinivas P, Sadanandam M. Formulation, evaluation and characterization of glipizide nanoemulsion. Asian J Pharm Clin Res. 2013,6:66-71.
Snyder LR, Kirkland JJ, Glajch JL. Practical HPLC Method Development, 2. ed. New Iork: Wiley Interscience, 765p, 1996.
Spiclin P, Gasperlin M, Kmetec V. Stability of ascorbyl palmitate in topical microemulsions. Int J Pharm. 2001;222:271-279.
Stamford NPJ. Stability, transdermal penetration, and cutaneous effects of ascorbic acid and its derivatives. J Cosmet Dermatol. 2012;11(4):310-7.
Suzuki T, Takei H, YamazAki S. Formation of fine three-phase emulsions by liquid crystal Emulsification Method with Arginini ß-branched monoalkyl phosphate. 1988, J Coll Interf Sci. 1989;129(2):491-500.
Xu J, Richards H. Silicium compounds derived from ascorbic acid, PCT/FR00/02713 (2004).
Yamada K, Yamashita J, Todo H, Miyamoto K, Hashimoto S, Tokudome Y, Hashimoto F, Sugibayashi K. Preparation and Evaluation of Liquid-Crystal Formulations with Skin-permeation-enhancing. Abilities for Entrapped Drugs. J Oleo Sci.2011;60(1):31-40.
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